{"created":"2023-05-15T14:50:27.774418+00:00","id":69010,"links":{},"metadata":{"_buckets":{"deposit":"8137cffb-690f-4b8e-a46d-43139acd6fb0"},"_deposit":{"created_by":1,"id":"69010","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"69010"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00069010","sets":["10:28"]},"author_link":["677281","677280","677282"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2007-07-13","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We investigated the alteration of homologous recombination\nrepair in synchronous CHO cells irradiated with heavy ion particles\n(290MeV/n carbon ion, LET: 13keV/lm and 70keV/lm) and Xrays.\nThe synchronized cell populations were prepared by mitotic\nshake-off and sequential harvesting at various time points. Our flow\ncytometry analysis revealed that the majority of cells enter S-phase\nat 8 hours, and G2-phase at 12 hours after plating. We scored the\nnumber of co-localization sites of gamma-H2AX (DNA double\nstrand breaks) and Rad51 (homologous recombination repair\nprotein) foci after 1Gy irradiation. Carbon irradiated cells showed\na significantly higher number of co-localization sites at 30 minutes\nafter irradiation than X-irradiated cells. We also observed delayed\ndisappearance of Rad51 foci in carbon irradiated cells up to 2 hours.\nBoth chromosome- and chromatid-type aberrations were the highest\nin high LET carbon(70keV/lm), intermediate in low LET carbon\n(13keV/lm), and the lowest in x-irradiated cells. Furthermore, we\nobserved a significantly increased number of chromatid-type\naberrations in cells irradiated at early S-phase by 70 keV/lm\ncarbon ions. The cell survival rates by colony formation seem to\nreflect the chromosomal data as expected. We did not observe the\nresistant peak at late-S phase in cells irradiated with high LET (70\nkeV/lm) carbon ions. These results may indicate the alteration of\nhomologous recombination repair after high LET carbon irradiation\nlead to various biological effects. Our data are consistent with the\nidea of complex type DSB induced by high LET heavy ion\nirradiation.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"International Congress of Radiation Research","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kato, Takamitsu"}],"nameIdentifiers":[{"nameIdentifier":"677280","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"et.al"}],"nameIdentifiers":[{"nameIdentifier":"677281","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"加藤 宝光","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"677282","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"The effect of heavy-ions on synchronously dividing","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The effect of heavy-ions on synchronously dividing"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-07-17"},"publish_date":"2007-07-17","publish_status":"0","recid":"69010","relation_version_is_last":true,"title":["The effect of heavy-ions on synchronously dividing"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:19:33.302347+00:00"}