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Down regulation of BRCA2 enhances tumor radio-sensitivity through inhibition of homologous recombination repair
https://repo.qst.go.jp/records/68927
https://repo.qst.go.jp/records/68927b8a9b473-a3d4-4184-aeaf-c485f71af13c
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-04-22 | |||||
| タイトル | ||||||
| タイトル | Down regulation of BRCA2 enhances tumor radio-sensitivity through inhibition of homologous recombination repair | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Yu, Dong
× Yu, Dong× Sekine, Emiko× Fujimori, Akira× Okayasu, Ryuichi× 于 冬× 関根 絵美子× 藤森 亮× 岡安 隆一 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | In order to understand the role of BRCA2 protein in homologous recombination (HR) repair and radio-sensitization, we utilized RNA interference (RNAi) strategy to knock down the expression of this protein in HeLa cells. HR and non-homologous end joining (NHEJ) are the two major pathways for repair of DNA double strand breaks (DSBs) in mammalian cells. HR pathway is known to work in late-S and G2 phases of the cell cycle, while NHEJ does not seem to depend on the cell cycle. HeLa cells were transfected with BRCA2 siRNA (Qiagen) as well as negative-control siRNA for 48 hours. After confirming the downregulations of BRCA2 mRNA by quantitative RT-PCR and the protein level by western blotting, the radiosensitivity of transfected Hela cells was examined by colony formation assay and DNA DSB repair was measured by constant field gel-electrophoresis technique. Immuno-staining method was used to study the expression and phosphorylation of key DSB repair proteins such as Rad51 (HR pathway), DNA-PKcs (NHEJ pathway) and gamma-H2AX. Our data clearly demonstrate that downregulation of BRCA2 leads to radio-sensitization through inhibition of DSB repair. Since Rad51 foci formation was significantly affected by BRCA2 siRNA without affecting DNA-PKcs phosphorylation, BRCA2 appears to play a major role in the HR pathway, but not in the NHEJ pathway. These results suggest the possibility of using BRCA2 siRNA as a new radiosensitizer for circulating tumor cells. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | AACR Annual Meeting 2007 | |||||
| 発表年月日 | ||||||
| 日付 | 2007-04-18 | |||||
| 日付タイプ | Issued | |||||
