{"created":"2023-05-15T14:50:24.134241+00:00","id":68927,"links":{},"metadata":{"_buckets":{"deposit":"6991abad-f107-47ed-b628-61b660c27d76"},"_deposit":{"created_by":1,"id":"68927","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"68927"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00068927","sets":["10:28"]},"author_link":["676510","676509","676511","676516","676515","676513","676512","676514"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2007-04-18","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In order to understand the role of BRCA2 protein in homologous recombination (HR) repair and radio-sensitization, we utilized RNA interference (RNAi) strategy to knock down the expression of this protein in HeLa cells. HR and non-homologous end joining (NHEJ) are the two major pathways for repair of DNA double strand breaks (DSBs) in mammalian cells. HR pathway is known to work in late-S and G2 phases of the cell cycle, while NHEJ does not seem to depend on the cell cycle. HeLa cells were transfected with BRCA2 siRNA (Qiagen) as well as negative-control siRNA for 48 hours. After confirming the downregulations of BRCA2 mRNA by quantitative RT-PCR and the protein level by western blotting, the radiosensitivity of transfected Hela cells was examined by colony formation assay and DNA DSB repair was measured by constant field gel-electrophoresis technique. Immuno-staining method was used to study the expression and phosphorylation of key DSB repair proteins such as Rad51 (HR pathway), DNA-PKcs (NHEJ pathway) and gamma-H2AX. Our data clearly demonstrate that downregulation of BRCA2 leads to radio-sensitization through inhibition of DSB repair. Since Rad51 foci formation was significantly affected by BRCA2 siRNA without affecting DNA-PKcs phosphorylation, BRCA2 appears to play a major role in the HR pathway, but not in the NHEJ pathway. These results suggest the possibility of using BRCA2 siRNA as a new radiosensitizer for circulating tumor cells.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"AACR Annual Meeting 2007","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yu, Dong"}],"nameIdentifiers":[{"nameIdentifier":"676509","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sekine, Emiko"}],"nameIdentifiers":[{"nameIdentifier":"676510","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fujimori, Akira"}],"nameIdentifiers":[{"nameIdentifier":"676511","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okayasu, Ryuichi"}],"nameIdentifiers":[{"nameIdentifier":"676512","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"于 冬","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"676513","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"関根 絵美子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"676514","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"藤森 亮","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"676515","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岡安 隆一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"676516","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Down regulation of BRCA2 enhances tumor radio-sensitivity through inhibition of homologous recombination repair","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Down regulation of BRCA2 enhances tumor radio-sensitivity through inhibition of homologous recombination repair"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-04-22"},"publish_date":"2007-04-22","publish_status":"0","recid":"68927","relation_version_is_last":true,"title":["Down regulation of BRCA2 enhances tumor radio-sensitivity through inhibition of homologous recombination repair"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:20:28.867089+00:00"}