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マウスmonocyte-macrophage細胞株RAW264.7における、15-deoxy-delta 12,14-prostaglandin J2による、PPARγ非依存性のHO-1転写活性化
https://repo.qst.go.jp/records/68541
https://repo.qst.go.jp/records/68541b3100b01-3b0f-4817-99f8-caeba0ae8c84
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2006-06-26 | |||||
| タイトル | ||||||
| タイトル | マウスmonocyte-macrophage細胞株RAW264.7における、15-deoxy-delta 12,14-prostaglandin J2による、PPARγ非依存性のHO-1転写活性化 | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
鈴木, 桂子
× 鈴木, 桂子× 田中, 泉× 榑松, 文子× 石原, 弘× 鈴木 桂子× 田中 泉× 槫松 文子× 石原 弘 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Prostaglandin J2 analog is known to have cytoprotective activities. Especially in our body 15-deoxy-delta 12,14-prostaglandin J2 (15d-PGJ2) is produced and acting as a cytoprotector. We examined whether 15d-PGJ2 activates heme oxygenase-1 (HO-1) gene in mouse monocyte-macrophage cell line, RAW 264.7. Cells were treated with 15d-PGJ2 for 4 h in DMEM. mRNAs of HO-1 and GAPDH were estimated by the RT-PCR method. As a result we detected a 105 times increase of HO-1 mRNA with 500 nM 15d-PGJ2. We have investigated whether PPARgamma is involved in this activation. At first we examined the effect of troglitazone which is an agonist of PPARgamma. We could not detect any increase of HO-1 mRNA by troglitazone. Therefore it was confirmed that the activation of PPARgamma does not activate the transcription of HO-1 gene. We next examined the effect of GW9662, which is an antagonist of PPARgamma, upon transcription of HO-1 gene. GW9662 did not affect the mRNA of HO-1 gene. From these results we conclude that the transcriptional activation of HO-1 gene by 15d-PGJ2 is not mediated by PPARgamma. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 第78回日本生化学会大会 | |||||
| 発表年月日 | ||||||
| 日付 | 2005-10-22 | |||||
| 日付タイプ | Issued | |||||
