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Combined effects of X-rays and N-ethyl-N-nitrosourea in murine thymic lymphoma s -(2) Analysis in Ikaros point mutation-
https://repo.qst.go.jp/records/68243
https://repo.qst.go.jp/records/68243dc018c2e-7399-4492-adb6-85bc276ca0a7
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2005-11-19 | |||||
| タイトル | ||||||
| タイトル | Combined effects of X-rays and N-ethyl-N-nitrosourea in murine thymic lymphoma s -(2) Analysis in Ikaros point mutation- | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Amasaki, Yoshiko
× Amasaki, Yoshiko× Kakinuma, Shizuko× Yamauchi, Kazumi× Nishimura, Mayumi× Imaoka, Tatsuhiko× Shimada, Yoshiya× 甘崎 佳子× 柿沼 志津子× 山内 一己× 西村 まゆみ× 今岡 達彦× 島田 義也 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Ikaros is a potent tumor suppressor gene in both human and murine leukemogenesis. We previously showed Ikaros alteration type is differed between X-ray-induced murine thymic lymphoma (TL) and N-ethyl-N-nitrosourea (ENU) -induced TL. Thus, X-ray-induced TL was characterized by altered splicing, null expression and point mutations, which were accompanied by by loss of heterozygosity (LOH). ENU-induced TL harbored only point mutation without LOH. We showed that combined treatment of X-rays (0.8-1.0 Gy x 4 times) and ENU (100, 200ppm) synergistically increased TL incidence. The aim of the curresnt study is to elucidate the underlying mechanism in the lymphomagenesis induced by combined treatment of X-rays and ENU. In this study, we analyzed the mutation status of Ikaros in the TLs induced by combined (X-rays following ENU) treatment. B6C3F1 mice (4-week-old female) were irradiated by X-rays (0.2 - 1.0 Gy a week x 4 times) and then treated by EMU (50 - 200 ppm in drinking water) from 8-week-old. The analyses of LOH on chromosome 11, expression by RT-PCR and western blotting, and mutation by sequence were performed. Ikaros splicing and null expression was observed in X-ray-induced TL was 10 and12%, ENU-induced TL was both 0%, combined-induced TL was 6 and 0%. Point mutation and LOH status were 26%(LOH:73%) in X-ray-induced TL 26%(LOH:0%) in ENU-induced TL. In combined-induced TL, that was increased to 40% (LOH: 20%), it is ENU type. Thus, X-rays plays a promoter for ENU-induced leukemogenesis in the TL induced by X-rays following ENU. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 第48回日本放射線影響学会／第1回アジア放射線研究会議 | |||||
| 発表年月日 | ||||||
| 日付 | 2005-11-17 | |||||
| 日付タイプ | Issued | |||||
