{"created":"2023-05-15T14:49:50.829962+00:00","id":68243,"links":{},"metadata":{"_buckets":{"deposit":"aa32c0a0-37ee-4360-a3a2-a5d8023d6a3b"},"_deposit":{"created_by":1,"id":"68243","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"68243"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00068243","sets":["10:28"]},"author_link":["670000","670004","670006","670002","670003","669995","669999","669998","669996","670005","670001","669997"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2005-11-17","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Ikaros is a potent tumor suppressor gene in both human and murine leukemogenesis.  We previously showed Ikaros alteration type is differed between X-ray-induced murine thymic lymphoma (TL) and N-ethyl-N-nitrosourea (ENU) -induced TL. Thus, X-ray-induced TL was characterized by altered splicing, null expression and point mutations, which were accompanied by by loss of heterozygosity (LOH). ENU-induced TL harbored only point mutation without LOH. We showed that combined treatment of X-rays (0.8-1.0 Gy x 4 times) and ENU (100, 200ppm) synergistically increased TL incidence. The aim of the curresnt study is to elucidate the underlying mechanism in the lymphomagenesis induced by combined treatment of X-rays and ENU. In this study, we analyzed the mutation status of Ikaros in the TLs induced by combined (X-rays following ENU) treatment.  \nB6C3F1 mice (4-week-old female) were irradiated by X-rays (0.2 - 1.0 Gy a week x 4 times) and then treated by EMU (50 - 200 ppm in drinking water) from 8-week-old. The analyses of LOH on chromosome 11, expression by RT-PCR and western blotting, and mutation by sequence were performed. \nIkaros splicing and null expression was observed in X-ray-induced TL was 10 and12%, ENU-induced TL was both 0%, combined-induced TL was 6 and 0%. Point mutation and LOH status were 26%(LOH:73%) in X-ray-induced TL 26%(LOH:0%) in ENU-induced TL. In combined-induced TL, that was increased to 40% (LOH: 20%), it is ENU type. Thus, X-rays plays a promoter for ENU-induced leukemogenesis in the TL induced by X-rays following ENU.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第48回日本放射線影響学会／第1回アジア放射線研究会議","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Amasaki, Yoshiko"}],"nameIdentifiers":[{"nameIdentifier":"669995","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kakinuma, Shizuko"}],"nameIdentifiers":[{"nameIdentifier":"669996","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamauchi, Kazumi"}],"nameIdentifiers":[{"nameIdentifier":"669997","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nishimura, Mayumi"}],"nameIdentifiers":[{"nameIdentifier":"669998","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Imaoka, Tatsuhiko"}],"nameIdentifiers":[{"nameIdentifier":"669999","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shimada, Yoshiya"}],"nameIdentifiers":[{"nameIdentifier":"670000","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"甘崎 佳子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"670001","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"柿沼 志津子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"670002","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"山内 一己","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"670003","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"西村 まゆみ","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"670004","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"今岡 達彦","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"670005","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"島田 義也","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"670006","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Combined effects of X-rays and N-ethyl-N-nitrosourea in murine thymic lymphoma s -(2) Analysis in Ikaros point mutation-","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Combined effects of X-rays and N-ethyl-N-nitrosourea in murine thymic lymphoma s -(2) Analysis in Ikaros point mutation-"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2005-11-19"},"publish_date":"2005-11-19","publish_status":"0","recid":"68243","relation_version_is_last":true,"title":["Combined effects of X-rays and N-ethyl-N-nitrosourea in murine thymic lymphoma s -(2) Analysis in Ikaros point mutation-"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:28:25.250018+00:00"}