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Study of DNA structural mutation induced by heavy charged particles (carbon ions) in head and neck malignant tumor cell lines
https://repo.qst.go.jp/records/68013
https://repo.qst.go.jp/records/680134b9affff-1259-4175-b372-3895af9e2c0b
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2005-02-08 | |||||
| タイトル | ||||||
| タイトル | Study of DNA structural mutation induced by heavy charged particles (carbon ions) in head and neck malignant tumor cell lines | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Yamamoto, Nobuharu
× Yamamoto, Nobuharu× Nakamoto, Daisuke× Numasawa, Hideyuki× Tanaka, Chihaya× Yakushiji, Takashi× Nomura, Takeshi× Uzawa, Katsuhiro× Shibahara, Takahiko× Tanzawa, Hideki× Mizoe, Junetsu× Noma, Hiroyasu× 山本 信治× 中本 大介× 柴原 孝彦× 溝江 純悦× 野間 弘康 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Heavy charged particle therapy achieves superior results for such non-squamous cell carcinoma as malignant melanoma and aenocarcinoma that are intractable to conventional photon radiation, rather than squamous cell carcinoma. In the absence of adequate geetic analysis at the molecular level, we have investigated the changes in genetic structure caused by the irradiation of heavy charged particles in cell stains from various types of malignant tumor tissue in the head and neck. Four types of cell lines, namely aquamous cell carcinoma(Ca9-22), malignant melanoma(G361), salivary gland carcinoma (HSG) and keatinocyte (HaCaT), were analyzed by irradiation with heavy charged particles (carbon ion radiation)(1, 4, 7GyE) and photons (1, 4, 8Gy).After irradiation, DNA was extracted (after 1, 24, and 48 hours) for analysis of the 6 microsatellite regions chromososome 17 using the PCR-LOH assay. After photon irradiation, LOH was extensively found at low radiation doese in squamous cell carcinoma cells after 48 hours and in adenocarcinoma cells after 24 and 48 hours. At high doses, however, LOH incidence was lower in all cell types. After heavy charged oartcle irradiation, an LOH pattern was detected only in one malignant melanoma site, with all other lesions showing loss of homozygosity. This indicates that heavy charged parrticles induse lethal changese in the DNA regardless of time and dose. Further research will determine the reproducibility of these results to establish the therapeutic effectiveness of heavy charged oarticles. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 第６回アジア口腔顎顔面外科学会/第４９回日本口腔外科学会総会 | |||||
| 発表年月日 | ||||||
| 日付 | 2004-10-23 | |||||
| 日付タイプ | Issued | |||||
