@misc{oai:repo.qst.go.jp:00068013,
 author = {Yamamoto, Nobuharu and Nakamoto, Daisuke and Numasawa, Hideyuki and Tanaka, Chihaya and Yakushiji, Takashi and Nomura, Takeshi and Uzawa, Katsuhiro and Shibahara, Takahiko and Tanzawa, Hideki and Mizoe, Junetsu and Noma, Hiroyasu and 山本 信治 and 中本 大介 and 柴原 孝彦 and 溝江 純悦 and 野間 弘康},
 month = {Oct},
 note = {Heavy charged particle therapy achieves superior results for such non-squamous cell carcinoma as malignant melanoma and aenocarcinoma that are intractable to conventional photon radiation, rather than squamous cell carcinoma. In the absence of adequate geetic analysis at the molecular level, we have investigated the changes in genetic structure caused by the irradiation of heavy charged particles in cell stains from various types of malignant tumor tissue in the head and neck. Four types of cell lines, namely aquamous cell carcinoma(Ca9-22), malignant melanoma(G361), salivary gland carcinoma (HSG) and keatinocyte (HaCaT), were analyzed by irradiation with heavy charged particles (carbon ion radiation)(1, 4, 7GyE) and photons (1, 4, 8Gy).After irradiation, DNA was extracted (after 1, 24, and 48 hours) for analysis of the 6 microsatellite regions chromososome 17 using the PCR-LOH assay. After photon irradiation, LOH was extensively found at low radiation doese in squamous cell carcinoma cells after 48 hours and in  adenocarcinoma cells after 24 and 48 hours. At high doses, however, LOH incidence was lower in all cell types. After heavy charged oartcle irradiation, an LOH pattern was detected only in one malignant melanoma site, with all other lesions showing loss of homozygosity. This indicates that heavy charged parrticles induse lethal changese in the DNA regardless of time and dose. Further research will determine the reproducibility of these results to establish the therapeutic effectiveness of heavy charged oarticles., 第６回アジア口腔顎顔面外科学会/第４９回日本口腔外科学会総会},
 title = {Study of DNA structural mutation induced by heavy charged particles (carbon ions) in head and neck malignant tumor cell lines},
 year = {2004}
}