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Peroxynitrite-mediated Single Tyrosine Nitration Induces a Functional Modification of Cytochrome c in an Electron Trasfer Reaction.
https://repo.qst.go.jp/records/67288
https://repo.qst.go.jp/records/672887f8f267b-3a80-4e63-8411-9ec42db4c58e
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2002-11-19 | |||||
| タイトル | ||||||
| タイトル | Peroxynitrite-mediated Single Tyrosine Nitration Induces a Functional Modification of Cytochrome c in an Electron Trasfer Reaction. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Nakagawa, Hidehiko
× Nakagawa, Hidehiko× Ooshima, Yukiko× Takusagawa, Mitsuko× Ikota, Nobuo× Takahashi, Yutaka× Shimizu, Shigeomi× Ozawa, Toshihiko× 中川 秀彦× 大島 由起子× 田草川 光子× 伊古田 暢夫× 高橋 豊× 小澤 俊彦 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The redox reaction of cytochrome c after modification with peroxynitrite under physiological conditions was investigated. Cytochrome c was treated with a bolus of synthetic peroxynitrite at a sub-millimolar concentration, and then subjected to reduction by superoxide and oxidation by hydrogen peroxide. The ability for the membrane potential formation in the mitochondrial respiratory chain was also evaluated. After the treatment with peroxynitrite, the cytochrome c molecule was mono-nitrated mainly at a tyrosine residue, using liquid chromatography-electrospray ionizing mass spectrometry (LC-ESIMS) and HPLC. Although the redox capacity of cytochrome c was not affected by the peroxynitrite treatment, the oxidation of ferrocytochrome c to ferricytochrome c by hydrogen peroxide was accelerated. When cytochrome c was treated with peroxynitrite in the presence of 5-methoxytryptamine, an inhibitor for the tyrosine nitration by peroxynitrite, the acceleration of hydrogen peroxide-mediated oxidation was suppressed. It was also found that the formation of membrane potential in the rat liver mitochondria was suppressed when peroxynitrite-treated cytochrome c was used instead of the intact cytochrome c in vitro. From these results, we concluded that the peroxynitrite-treated cytochrome c was nitrated at a tyrosine residue and became more susceptible to oxidation by hydrogen peroxide, concomitantly losing the ability to transfer electrons ability in the mitochondrial respiratory chain. It is suggested that the peroxynitrite-induced modification of cytochrome c increases the susceptibility to non-physiological oxidants, and may cause dysfunction of mitochondria by suppressing of membrane potential. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | SFRR Australasia and SFRR Japan Joint Meeting | |||||
| 発表年月日 | ||||||
| 日付 | 2001-12-04 | |||||
| 日付タイプ | Issued | |||||
