@misc{oai:repo.qst.go.jp:00067288,
 author = {Nakagawa, Hidehiko and Ooshima, Yukiko and Takusagawa, Mitsuko and Ikota, Nobuo and Takahashi, Yutaka and Shimizu, Shigeomi and Ozawa, Toshihiko and 中川 秀彦 and 大島 由起子 and 田草川 光子 and 伊古田 暢夫 and 高橋 豊 and 小澤 俊彦},
 month = {Dec},
 note = {The redox reaction of cytochrome c after modification with peroxynitrite under physiological conditions was investigated. Cytochrome c was treated with a bolus of synthetic peroxynitrite at a sub-millimolar concentration, and then subjected to reduction by superoxide and oxidation by hydrogen peroxide. The ability for the membrane potential formation in the mitochondrial respiratory chain was also evaluated. After the treatment with peroxynitrite, the cytochrome c molecule was mono-nitrated mainly at a tyrosine residue, using liquid chromatography-electrospray ionizing mass spectrometry (LC-ESIMS) and HPLC. Although the redox capacity of cytochrome c was not affected by the peroxynitrite treatment, the oxidation of ferrocytochrome c to ferricytochrome c by hydrogen peroxide was accelerated. When cytochrome c was treated with peroxynitrite in the presence of 5-methoxytryptamine, an inhibitor for the tyrosine nitration by peroxynitrite, the acceleration of hydrogen peroxide-mediated oxidation was suppressed. It was also found that the formation of membrane potential in the rat liver mitochondria was suppressed when peroxynitrite-treated cytochrome c was used instead of the intact cytochrome c in vitro. From these results, we concluded that the peroxynitrite-treated cytochrome c was nitrated at a tyrosine residue and became more susceptible to oxidation by hydrogen peroxide, concomitantly losing the ability to transfer electrons ability in the mitochondrial respiratory chain. It is suggested that the peroxynitrite-induced modification of cytochrome c increases the susceptibility to non-physiological oxidants, and may cause dysfunction of mitochondria by suppressing of membrane potential., SFRR Australasia and SFRR Japan Joint Meeting},
 title = {Peroxynitrite-mediated Single Tyrosine Nitration Induces a Functional Modification of Cytochrome c in an Electron Trasfer Reaction.},
 year = {2001}
}