WEKO3
アイテム
Bystander WI-38 normal lung fibroblast cells modulate DNA double-strand break repair in microbeam-targeted A549 cells through gap junction intercellular communication
https://repo.qst.go.jp/records/66525
https://repo.qst.go.jp/records/66525d4fb5e98-3cb0-4c1b-9ebc-461d2ebf9485
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2017-11-22 | |||||
| タイトル | ||||||
| タイトル | Bystander WI-38 normal lung fibroblast cells modulate DNA double-strand break repair in microbeam-targeted A549 cells through gap junction intercellular communication | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Kobayashi, Alisa
× Kobayashi, Alisa× Autsavapromporn, Narongchai× Ahbrizal, Farizal Tengku Ahmad Tengku× Oikawa, Masakazu× Homma-Takeda, Shino× Furusawa, Yoshiya× Wang, Jun× Konishi, Teruaki× 小林 亜利紗× 及川 将一× 武田 志乃× 古澤 佳也× 小西 輝昭 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The mechanisms underlying bi-directional signaling involved in radiation-induced bystander effect (RIBE) between irradiated carcinoma cells and their surrounding non-irradiated normal cells is relevant to radiation cancer therapy. Using the SPICE-NIRS microbeam, we delivered 500 protons to A549-GFP lung carcinoma cells, stably expressing H2B-GFP, that were co-cultured with normal WI-38 cells. The level of γ-H2AX, a marker for DNA double-strand breaks (DSB), was subsequently measured up to 24 h post irradiation in both targeted and bystander cells. As a result, gap junction intercellular communication (GJIC) inhibition attenuated DSB repair in targeted A549-GFP cells, and RIBE was suppressed in bystander WI-38 cells but not in distant A549-GFP cells. This suggested that GJIC plays a two-way role either propagating DNA damage effect between carcinoma to normal cells, or reversing the bystander signaling, also called a “rescue effect” from bystander cells to irradiated cells, in order to enhance the DSB repair in targeted cells. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | MICROS 2017 17th International Symposium on Microdosimetry | |||||
| 発表年月日 | ||||||
| 日付 | 2017-11-07 | |||||
| 日付タイプ | Issued | |||||
