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Late Effects in the Progeny of Bystander Human Cells after Carbon Ions are Dependent on Radiation Quality: The Relevance to Cancer Risk
https://repo.qst.go.jp/records/66095
https://repo.qst.go.jp/records/66095bd545dea-f57c-44d2-bd1c-57fbf45df788
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2017-01-28 | |||||
| タイトル | ||||||
| タイトル | Late Effects in the Progeny of Bystander Human Cells after Carbon Ions are Dependent on Radiation Quality: The Relevance to Cancer Risk | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Autsavapromporn, Narongchai
× Autsavapromporn, Narongchai× Konishi, Teruaki× Liu, Cuihua× I, Azzam Edourd× Plante, Ianik× Funayama, Tomoo× Suzuki, Masao× 小西 輝昭× 劉 翠華× 舟山 知夫× 鈴木 雅雄 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Ionizing Radiation (IR) is an importantly modality in the treatment of many types of human cancer. In particular, heavy ions such as carbon ions offer improved dose distribution and reduced the side effects in normal cells near the tumors, compared with conventional radiotherapy (X rays). On the other hand, IR-induced bystander effects and genomic instability have implication in human health, which may lead to carcinogenesis through mutation induction. However, the underlying mechanisms of cancer risks in normal cells following carbon ion irradiation is widely unknown. The objective of this study is to investigate the role of radiation quality and gap-junction intercellular communication (GJIC) in the propagation of stressful effects in the progeny of normal human skin fibroblasts cultures. Briefly, confluent cells in the presence and absence of gap-junction inhibitor AGA were exposed to carbon ions with a different linear energy transfer (LET) either 290 MeV/u carbon (LET 76 keV/m) using a transwell insert co-culture system or 18.3 MeV/u carbon microbeam (LET 103 keV/m) at mean absorbed dose 0.4-6 Gy, wherein 0-0.4% of the cells were targeted by IR. Following 20 populations post-irradiation, the progeny of bystander normal cells were harvested and assayed for several of biological endpoints. Our results showed that expression of stressful effects in the progeny of bystander cells is dependent on LET. The progeny of bystander cells exposed to low-LET carbon ions showed the persistence of oxidative stress and correlate with the increased micronucleus formation and mutant fraction. Such effect were not observed after high-LET carbon ion exposures. Interestingly, inhibition of GJIC mitigated the toxic effects in the progeny of bystander cells. Together, this results contributes to understanding of fundamental radiation biology relating to the high LET carbon ions can reduce cancer risk after radiotherapy and GJIC may be a critical mediator in the observed effects. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | International Nuclear Science and Technology Conference 2016 | |||||
| 発表年月日 | ||||||
| 日付 | 2016-08-05 | |||||
| 日付タイプ | Issued | |||||
