@misc{oai:repo.qst.go.jp:00066095,
 author = {Autsavapromporn, Narongchai and Konishi, Teruaki and Liu, Cuihua and I, Azzam Edourd and Plante, Ianik and Funayama, Tomoo and Suzuki, Masao and 小西 輝昭 and 劉 翠華 and 舟山 知夫 and 鈴木 雅雄},
 month = {Aug},
 note = {Ionizing Radiation (IR) is an importantly modality in the treatment of many types of human cancer.  In particular, heavy ions such as carbon ions offer improved dose distribution and reduced the side effects in normal cells near the tumors, compared with conventional radiotherapy (X rays). On the other hand, IR-induced bystander effects and genomic instability have implication in human health, which may lead to carcinogenesis through mutation induction. However, the underlying mechanisms of cancer risks in normal cells following carbon ion irradiation is widely unknown. The objective of this study is to investigate the role of radiation quality and gap-junction intercellular communication (GJIC) in the propagation of stressful effects in the progeny of normal human skin fibroblasts cultures. Briefly, confluent cells in the presence and absence of gap-junction inhibitor AGA were exposed to carbon ions with a different linear energy transfer (LET) either 290 MeV/u carbon (LET 76 keV/m) using a transwell insert co-culture system or 18.3 MeV/u carbon microbeam (LET 103 keV/m) at mean absorbed dose 0.4-6 Gy, wherein 0-0.4% of the cells were targeted by IR. Following 20 populations post-irradiation, the progeny of bystander normal cells were harvested and assayed for several of biological endpoints. Our results showed that expression of stressful effects in the progeny of bystander cells is dependent on LET. The progeny of bystander cells exposed to low-LET carbon ions showed the persistence of oxidative stress and correlate with the increased micronucleus formation and mutant fraction. Such effect were not observed after high-LET carbon ion exposures. Interestingly, inhibition of GJIC mitigated the toxic effects in the progeny of bystander cells. Together, this results contributes to understanding of fundamental radiation biology relating to the high LET carbon ions can reduce cancer risk after radiotherapy and GJIC may be a critical mediator in the observed effects., International Nuclear Science and Technology Conference 2016},
 title = {Late Effects in the Progeny of Bystander Human  Cells after Carbon Ions are Dependent on  Radiation Quality: The Relevance to  Cancer Risk},
 year = {2016}
}