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Neuronal secretory pathway essential for development and maintenance of brain circuit and synaptic function
https://repo.qst.go.jp/records/65827
https://repo.qst.go.jp/records/65827b717d387-fc5e-4a38-aa68-d11478ef90d7
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2015-12-10 | |||||
| タイトル | ||||||
| タイトル | Neuronal secretory pathway essential for development and maintenance of brain circuit and synaptic function | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Shimojyo, Masafumi
× Shimojyo, Masafumi× Courchet, Julien× Pieraut, Simon× Torabi-Rander, Nina× Sando, Richard× Polleux, Franck× Higuchi, Makoto× Maximov, Anton× 下條 雅文× 樋口 真人 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Neurons employ a diverse repertoire of trafficking organelles for secretion of soluble molecules and recycling of membrane components, and these mechanisms are essential for development and maintenance of brain environment. Recent studies have identified the core components of a secretory apparatus that triggers the release of neurotransmitters from presynaptic terminals. Synaptic vesicle exocytosis is mediated by SNARE proteins, Synaptobrevin/VAMP2 (Syb2), SNAP25 and Syntaxin1, whose assembly into ternary complexes facilitate membrane fusion. A variety of diffusible peptide cues also appear to be transported by vesicles and undergo exocytosis, supporting the hypothesis that diverse SNAREs independently control different type of membrane fusion. Among such peptide cues, the brain-derived neurotrophic factor (BDNF) is known to play many critical roles in the nervous system. However, the molecular mechanisms that control the secretion of BDNF and other diffusible protein cues remain largely unknown. Here, we demonstrate that, using total internal reflection fluorescence microscopy and BDNF fused to pH-sensitive GFP variant pHluorin, activity-dependent exocytosis of BDNF from somatodendrites and axons is mediated by SNARE proteins Syb2 and SNAP25, suggesting these SNAREs act in multiple secretory pathways. Importantly, axonal secretion of BDNF is also specifically regulated by SNAP47. Cell-autonomous shRNA mediated knockdown of SNAP47 impairs the targeting and terminal branching of callosal axons in vivo, similar to knockdown of BDNF and its receptor TrkB. Taken together, these novel SNARE functions in BDNF secretion provide an important insight for neuronal circuit connectivity during brain development and/or maintenance. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 新学術領域 脳内環境 班会議 第2回 若手研究者シンポジウム | |||||
| 発表年月日 | ||||||
| 日付 | 2015-01-08 | |||||
| 日付タイプ | Issued | |||||
