{"created":"2023-05-15T14:48:01.817906+00:00","id":65827,"links":{},"metadata":{"_buckets":{"deposit":"61b6f9d8-a3c4-40d0-a88c-27bc3eedfcad"},"_deposit":{"created_by":1,"id":"65827","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"65827"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00065827","sets":["10:29"]},"author_link":["648463","648465","648469","648468","648466","648470","648471","648464","648472","648467"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2015-01-08","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Neurons employ a diverse repertoire of trafficking organelles for secretion of soluble\nmolecules and recycling of membrane components, and these mechanisms are essential\nfor development and maintenance of brain environment. Recent studies have identified the\ncore components of a secretory apparatus that triggers the release of neurotransmitters\nfrom presynaptic terminals. Synaptic vesicle exocytosis is mediated by SNARE proteins,\nSynaptobrevin/VAMP2 (Syb2), SNAP25 and Syntaxin1, whose assembly into ternary\ncomplexes facilitate membrane fusion. A variety of diffusible peptide cues also appear to\nbe transported by vesicles and undergo exocytosis, supporting the hypothesis that diverse\nSNAREs independently control different type of membrane fusion. Among such peptide\ncues, the brain-derived neurotrophic factor (BDNF) is known to play many critical roles in\nthe nervous system. However, the molecular mechanisms that control the secretion of\nBDNF and other diffusible protein cues remain largely unknown.\nHere, we demonstrate that, using total internal reflection fluorescence microscopy and\nBDNF fused to pH-sensitive GFP variant pHluorin, activity-dependent exocytosis of\nBDNF from somatodendrites and axons is mediated by SNARE proteins Syb2 and\nSNAP25, suggesting these SNAREs act in multiple secretory pathways. Importantly,\naxonal secretion of BDNF is also specifically regulated by SNAP47. Cell-autonomous\nshRNA mediated knockdown of SNAP47 impairs the targeting and terminal branching of\ncallosal axons in vivo, similar to knockdown of BDNF and its receptor TrkB. Taken\ntogether, these novel SNARE functions in BDNF secretion provide an important insight\nfor neuronal circuit connectivity during brain development and/or maintenance.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"新学術領域 脳内環境 班会議 第2回 若手研究者シンポジウム","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Shimojyo, Masafumi"}],"nameIdentifiers":[{"nameIdentifier":"648463","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Courchet, Julien"}],"nameIdentifiers":[{"nameIdentifier":"648464","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Pieraut, Simon"}],"nameIdentifiers":[{"nameIdentifier":"648465","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Torabi-Rander, Nina"}],"nameIdentifiers":[{"nameIdentifier":"648466","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sando, Richard"}],"nameIdentifiers":[{"nameIdentifier":"648467","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Polleux, Franck"}],"nameIdentifiers":[{"nameIdentifier":"648468","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Higuchi, Makoto"}],"nameIdentifiers":[{"nameIdentifier":"648469","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Maximov, Anton"}],"nameIdentifiers":[{"nameIdentifier":"648470","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"下條 雅文","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"648471","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"樋口 真人","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"648472","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Neuronal secretory pathway essential for development and maintenance of brain circuit and synaptic function","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Neuronal secretory pathway essential for development and maintenance of brain circuit and synaptic function"}]},"item_type_id":"10005","owner":"1","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-12-10"},"publish_date":"2015-12-10","publish_status":"0","recid":"65827","relation_version_is_last":true,"title":["Neuronal secretory pathway essential for development and maintenance of brain circuit and synaptic function"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:55:49.994596+00:00"}