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HEAVY ION IRRADIATION ALONE OR IN COMBINATION WITH CHEMOTHRAPY EFFECTIVELY DISRUPTS PUTATIVE PANCREATIC CANCER STEM CELLS
https://repo.qst.go.jp/records/65735
https://repo.qst.go.jp/records/657356c31e81f-b4d1-4e17-8f25-141f0378378a
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2015-09-03 | |||||
| タイトル | ||||||
| タイトル | HEAVY ION IRRADIATION ALONE OR IN COMBINATION WITH CHEMOTHRAPY EFFECTIVELY DISRUPTS PUTATIVE PANCREATIC CANCER STEM CELLS | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
崔, 星
× 崔, 星× Ｇｕｉｌｌａｕｍｅ, Ｖａｒｅｓ× 山田, 滋× 崔 星× Ｇｕｉｌｌａｕｍｅ Ｖａｒｅｓ× 山田 滋 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | To examine different effects of carbon ion beam alone or in combination with chemotherapy on putative pancreatic cancer stem cell (CSC) survival and DNA repair in comparison with X-ray, human pancreatic CSCs sorted from PANC1 and PK45 cells were treated with carbon ion beam, X-ray alone or in combination with gemcitabine. We found that the number of colony and spheroid formed from CSCs (CD44+/ESA+) after carbon ion beam was significantly reduced compared to that of X-ray, and it was extremely suppressed when combined with gemcitabine or 5-FU. The relative biological effectiveness (RBE) values for the carbon ion beam relative to X-ray at the D10 levels for CSCs were 2.23-2.66. RT Profiler PCR Array analysis showed that expressions of apoptosis-related genes (Bax, Cytochrome c), autophagy-related genes (LC3, p62), and senescence-related gene p21 were remarkably induced after carbon ion beam combined with gemcitabine compared to carbon ion beam alone or X-ray combined with gemcitabine. Not only the number but also the size of γH2AX foci in CSCs were lager 24 h after carbon ion beam combined with gemcitabine or 5-FU compared to carbon ion beam alone or X-ray combined with gemcitabine. Xenograft tumor control analysis showed that the tumors were not completely controlled even treated with 60 Gy of X-ray, but it was destroyed with 25 Gy of carbon ion beam combined with 50 mg/kg gemcitabine. In conclusion, carbon ion beam combined with gemcitabine or 5-FU has superior potential to kill pancreatic CSCs via unrepairable clustered DSB, and multiple cell death pathways. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 19th International Association of Pancreatology (IAP) Annual Meeting | |||||
| 発表年月日 | ||||||
| 日付 | 2015-08-29 | |||||
| 日付タイプ | Issued | |||||
