{"created":"2023-05-15T14:47:57.750693+00:00","id":65735,"links":{},"metadata":{"_buckets":{"deposit":"30e8abb9-da61-48d8-9a68-789912e954a5"},"_deposit":{"created_by":1,"id":"65735","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"65735"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00065735","sets":["10:29"]},"author_link":["647459","647461","647463","647458","647460","647462"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2015-08-29","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"To examine different effects of carbon ion beam alone or in combination with chemotherapy on putative pancreatic cancer stem cell (CSC) survival and DNA repair in comparison with X-ray, human pancreatic CSCs sorted from PANC1 and PK45 cells were treated with carbon ion beam, X-ray alone or in combination with gemcitabine. We found that the number of colony and spheroid formed from CSCs (CD44+/ESA+) after carbon ion beam was significantly reduced compared to that of X-ray, and it was extremely suppressed when combined with gemcitabine or 5-FU. The relative biological effectiveness (RBE) values for the carbon ion beam relative to X-ray at the D10 levels for CSCs were 2.23-2.66. RT Profiler PCR Array analysis showed that expressions of apoptosis-related genes (Bax, Cytochrome c), autophagy-related genes (LC3, p62), and senescence-related gene p21 were remarkably induced after carbon ion beam combined with gemcitabine compared to carbon ion beam alone or X-ray combined with gemcitabine. Not only the number but also the size of γH2AX foci in CSCs were lager 24 h after carbon ion beam combined with gemcitabine or 5-FU compared to carbon ion beam alone or X-ray combined with gemcitabine. Xenograft tumor control analysis showed that the tumors were not completely controlled even treated with 60 Gy of X-ray, but it was destroyed with 25 Gy of carbon ion beam combined with 50 mg/kg gemcitabine. In conclusion, carbon ion beam combined with gemcitabine or 5-FU has superior potential to kill pancreatic CSCs via unrepairable clustered DSB, and multiple cell death pathways.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"19th International Association of Pancreatology (IAP) Annual Meeting","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"崔, 星"}],"nameIdentifiers":[{"nameIdentifier":"647458","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ｇｕｉｌｌａｕｍｅ, Ｖａｒｅｓ"}],"nameIdentifiers":[{"nameIdentifier":"647459","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"山田, 滋"}],"nameIdentifiers":[{"nameIdentifier":"647460","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"崔 星","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"647461","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ｇｕｉｌｌａｕｍｅ Ｖａｒｅｓ","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"647462","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"山田 滋","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"647463","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"HEAVY ION IRRADIATION ALONE OR IN COMBINATION WITH CHEMOTHRAPY EFFECTIVELY DISRUPTS PUTATIVE PANCREATIC CANCER STEM CELLS","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"HEAVY ION IRRADIATION ALONE OR IN COMBINATION WITH CHEMOTHRAPY EFFECTIVELY DISRUPTS PUTATIVE PANCREATIC CANCER STEM CELLS"}]},"item_type_id":"10005","owner":"1","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-09-03"},"publish_date":"2015-09-03","publish_status":"0","recid":"65735","relation_version_is_last":true,"title":["HEAVY ION IRRADIATION ALONE OR IN COMBINATION WITH CHEMOTHRAPY EFFECTIVELY DISRUPTS PUTATIVE PANCREATIC CANCER STEM CELLS"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:56:51.495794+00:00"}