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Radioprotective effects of FGF1/CPPC fusion proteins
https://repo.qst.go.jp/records/64881
https://repo.qst.go.jp/records/64881dab1d3ef-7c63-4c95-bd58-4180acbd2575
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-12-17 | |||||
| タイトル | ||||||
| タイトル | Radioprotective effects of FGF1/CPPC fusion proteins | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Nakayama, Fumiaki
× Nakayama, Fumiaki× Umeda, Sachiko× Yasuda, Takeshi× Asada, Masahiro× Imamura, Toru× Imai, Takashi× 中山 文明× 梅田 禎子× 安田 武嗣× 今井 高志 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Although FGF11 subfamily members (FGF11-14) have structural similarity with FGF1, a significant structural difference between FGF11-14 and FGF1 is the C-terminal sequence because FGF11-14 possess unique C-terminal polypeptides. We identified novel cell-penetrating peptide domains (CPPC11-14) from the C-terminal region of FGF11-14 proteins, which could readily deliver FGF11-14 into cells independently of FGF receptors (FGFRs). This study evaluated and compared the protective activity of FGF1/CPPC fusion proteins (FGF1/CPPC11-14) and FGF1 against radiation damage. The in vitro mitogenic activity of FGF1/CPPC12 was examined by a cell growth assay using a BaF3 transfectant cell line expressing the FGFR1c subtype (BaF3-FGFR1c). FGF1/CPPC12 was less potent than FGF1 for mitogenic activity through FGFR1c regardless of whether heparin was added or not. In contrast, FGF1/CPPC12 significantly reduced radiation-induced apoptosis in the rat intestinal epithelial cell line IEC6 in the presence of heparin. The protective activity of FGF1/CPPC12 against radiation-induced intestinal injuries was evaluated in BALB/c mice. As a result, FGF1/CPPC12 significantly enhanced crypt survival compared with FGF1 even in the absence of heparin and FGF1/CPPC12-treatment significantly increased BrdU incorporation into the crypts and the depth of the crypts more than FGF1. These findings indicate that FGF1/CPPC fusion proteins strongly protect the jejunum against radiation-induced injury and suggest that cellular internalization of FGF1/CPPC11-14 is involved in their activities independently of FGFRs. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The 35th Annual Meeting of the Molecular Biology Society of Japan | |||||
| 発表年月日 | ||||||
| 日付 | 2012-12-14 | |||||
| 日付タイプ | Issued | |||||
