{"created":"2023-05-15T14:47:18.834196+00:00","id":64881,"links":{},"metadata":{"_buckets":{"deposit":"86e914eb-3c9d-4c2d-916d-0f96a4f57d52"},"_deposit":{"created_by":1,"id":"64881","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"64881"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00064881","sets":["10:29"]},"author_link":["639433","639434","639426","639430","639431","639429","639428","639425","639427","639432"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2012-12-14","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Although FGF11 subfamily members (FGF11-14) have structural similarity with FGF1, a significant structural difference between FGF11-14 and FGF1 is the C-terminal sequence because FGF11-14 possess unique C-terminal polypeptides. We identified novel cell-penetrating peptide domains (CPPC11-14) from the C-terminal region of FGF11-14 proteins, which could readily deliver FGF11-14 into cells independently of FGF receptors (FGFRs). This study evaluated and compared the protective activity of FGF1/CPPC fusion proteins (FGF1/CPPC11-14) and FGF1 against radiation damage. The in vitro mitogenic activity of FGF1/CPPC12 was examined by a cell growth assay using a BaF3 transfectant cell line expressing the FGFR1c subtype (BaF3-FGFR1c). FGF1/CPPC12 was less potent than FGF1 for mitogenic activity through FGFR1c regardless of whether heparin was added or not. In contrast, FGF1/CPPC12 significantly reduced radiation-induced apoptosis in the rat intestinal epithelial cell line IEC6 in the presence of heparin. The protective activity of FGF1/CPPC12 against radiation-induced intestinal injuries was evaluated in BALB/c mice. As a result, FGF1/CPPC12 significantly enhanced crypt survival compared with FGF1 even in the absence of heparin and FGF1/CPPC12-treatment significantly increased BrdU incorporation into the crypts and the depth of the crypts more than FGF1. These findings indicate that FGF1/CPPC fusion proteins strongly protect the jejunum against radiation-induced injury and suggest that cellular internalization of FGF1/CPPC11-14 is involved in their activities independently of FGFRs.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"The 35th Annual Meeting of the Molecular Biology Society of Japan","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nakayama, Fumiaki"}],"nameIdentifiers":[{"nameIdentifier":"639425","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Umeda, Sachiko"}],"nameIdentifiers":[{"nameIdentifier":"639426","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yasuda, Takeshi"}],"nameIdentifiers":[{"nameIdentifier":"639427","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Asada, Masahiro"}],"nameIdentifiers":[{"nameIdentifier":"639428","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Imamura, Toru"}],"nameIdentifiers":[{"nameIdentifier":"639429","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Imai, Takashi"}],"nameIdentifiers":[{"nameIdentifier":"639430","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"中山 文明","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"639431","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"梅田 禎子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"639432","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"安田 武嗣","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"639433","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"今井 高志","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"639434","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Radioprotective effects of FGF1/CPPC fusion proteins","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Radioprotective effects of FGF1/CPPC fusion proteins"}]},"item_type_id":"10005","owner":"1","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-12-17"},"publish_date":"2012-12-17","publish_status":"0","recid":"64881","relation_version_is_last":true,"title":["Radioprotective effects of FGF1/CPPC fusion proteins"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T21:06:31.136945+00:00"}