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However, the relationship between changes to arterial resistance (i.e., vasodilation) and the resultant increases of cerebral blood flow (CBF) remains incompletely understood. In the present study, we measured changes in the diameter of arteries on the cortical surface and in parenchymal tissue induced by sensory stimulation in awake mice. \nMethods: STOCK Tg[Tie2GFP] 287Sato/J mice (N = 7), in which the vascular endothelium had been genetically engineered to express green fluorescent protein, were used for the experiments with a chronic cranial window over the somatosensory cortex.  Sulforhodamine 101 was injected intraperitoneally to mice to fluorescently label blood plasma[1]. Cortical surface and parenchymal arteries at depths of 100, 200, 300 and 400 \u0026micro;m were scanned with either confocal or two-photon laser scanning microscopy (TCS SP5; Leica), and the arterial response to whisker stimulation (10Hz for 5 sec) was observed. 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  1. 学会発表・講演等
  2. 口頭発表

Measuring the vascular diameter of brain surface arteries and parenchymal arterioles in awake mouse

https://repo.qst.go.jp/records/64780
https://repo.qst.go.jp/records/64780
635a5ca0-2e0e-4bae-8b67-fb194b4f8a85
Item type 会議発表用資料 / Presentation(1)
公開日 2012-10-05
タイトル
タイトル Measuring the vascular diameter of brain surface arteries and parenchymal arterioles in awake mouse
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_c94f
資源タイプ conference object
アクセス権
アクセス権 metadata only access
アクセス権URI http://purl.org/coar/access_right/c_14cb
著者 Sekiguchi, Yuta

× Sekiguchi, Yuta

WEKO 638226

Sekiguchi, Yuta

Search repository
Masamoto, Kazuto

× Masamoto, Kazuto

WEKO 638227

Masamoto, Kazuto

Search repository
Takuwa, Hiroyuki

× Takuwa, Hiroyuki

WEKO 638228

Takuwa, Hiroyuki

Search repository
Kawaguchi, Hiroshi

× Kawaguchi, Hiroshi

WEKO 638229

Kawaguchi, Hiroshi

Search repository
Kanno, Iwao

× Kanno, Iwao

WEKO 638230

Kanno, Iwao

Search repository
Ito, Hiroshi

× Ito, Hiroshi

WEKO 638231

Ito, Hiroshi

Search repository
Tomita, Yutaka

× Tomita, Yutaka

WEKO 638232

Tomita, Yutaka

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Suzuki, Norihiro

× Suzuki, Norihiro

WEKO 638233

Suzuki, Norihiro

Search repository
Sudo, Ryo

× Sudo, Ryo

WEKO 638234

Sudo, Ryo

Search repository
Tanishita, Kazuo

× Tanishita, Kazuo

WEKO 638235

Tanishita, Kazuo

Search repository
関口 優太

× 関口 優太

WEKO 638236

en 関口 優太

Search repository
正本 和人

× 正本 和人

WEKO 638237

en 正本 和人

Search repository
田桑 弘之

× 田桑 弘之

WEKO 638238

en 田桑 弘之

Search repository
川口 拓之

× 川口 拓之

WEKO 638239

en 川口 拓之

Search repository
菅野 巖

× 菅野 巖

WEKO 638240

en 菅野 巖

Search repository
伊藤 浩

× 伊藤 浩

WEKO 638241

en 伊藤 浩

Search repository
冨田 裕

× 冨田 裕

WEKO 638242

en 冨田 裕

Search repository
抄録
内容記述タイプ Abstract
内容記述 Objectives: Neural activity-induced responses of the arteries on the cortical surface and parenchymal tissue have a critical role in balancing oxygen supply and demand in the brain. However, the relationship between changes to arterial resistance (i.e., vasodilation) and the resultant increases of cerebral blood flow (CBF) remains incompletely understood. In the present study, we measured changes in the diameter of arteries on the cortical surface and in parenchymal tissue induced by sensory stimulation in awake mice.
Methods: STOCK Tg[Tie2GFP] 287Sato/J mice (N = 7), in which the vascular endothelium had been genetically engineered to express green fluorescent protein, were used for the experiments with a chronic cranial window over the somatosensory cortex. Sulforhodamine 101 was injected intraperitoneally to mice to fluorescently label blood plasma[1]. Cortical surface and parenchymal arteries at depths of 100, 200, 300 and 400 µm were scanned with either confocal or two-photon laser scanning microscopy (TCS SP5; Leica), and the arterial response to whisker stimulation (10Hz for 5 sec) was observed. Laser-Doppler flowmetry (FLO-C1; OMEGA FLO) was used to evaluate evoked CBF around the vascular imaging site.
Results and Discussion: We observed that the CBF induced by whisker stimulation increased by 15 +/- 6% (N = 4). Mean arterial diameters during the resting state were 39 +/- 7 mum (N = 4), 19 +/- 1 mum (N = 4), 16 +/- 4 mum (N = 7), 17 +/- 4 mum (N = 9), and 14 +/- 3 mum (N = 9) at depths of 0, 100, 200, 300, and 400 mum, respectively. In response to whisker stimulation, the arterial diameter increased significantly (P < 0.01) by 8.8%, 9.9%, 10.9%, 9.2%, and 10.3% relative to the resting state diameters at depths of 0, 100, 200, 300, and 400 mum, respectively. No significant differences were observed across the cortical surface and parenchymal tissue (P = 0.29). In conclusion, we found that the arteries on the cortical surface and in parenchymal tissue within depths of 100 to 400 &micro;m similarly dilated during sensory stimulation in awake mouse brain.
会議概要(会議名, 開催地, 会期, 主催者等)
内容記述タイプ Other
内容記述 ISOTT2012
発表年月日
日付 2012-08-23
日付タイプ Issued
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