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Measuring the vascular diameter of brain surface arteries and parenchymal arterioles in awake mouse
https://repo.qst.go.jp/records/64780
https://repo.qst.go.jp/records/64780635a5ca0-2e0e-4bae-8b67-fb194b4f8a85
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-10-05 | |||||
| タイトル | ||||||
| タイトル | Measuring the vascular diameter of brain surface arteries and parenchymal arterioles in awake mouse | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Sekiguchi, Yuta
× Sekiguchi, Yuta× Masamoto, Kazuto× Takuwa, Hiroyuki× Kawaguchi, Hiroshi× Kanno, Iwao× Ito, Hiroshi× Tomita, Yutaka× Suzuki, Norihiro× Sudo, Ryo× Tanishita, Kazuo× 関口 優太× 正本 和人× 田桑 弘之× 川口 拓之× 菅野 巖× 伊藤 浩× 冨田 裕 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Objectives: Neural activity-induced responses of the arteries on the cortical surface and parenchymal tissue have a critical role in balancing oxygen supply and demand in the brain. However, the relationship between changes to arterial resistance (i.e., vasodilation) and the resultant increases of cerebral blood flow (CBF) remains incompletely understood. In the present study, we measured changes in the diameter of arteries on the cortical surface and in parenchymal tissue induced by sensory stimulation in awake mice. Methods: STOCK Tg[Tie2GFP] 287Sato/J mice (N = 7), in which the vascular endothelium had been genetically engineered to express green fluorescent protein, were used for the experiments with a chronic cranial window over the somatosensory cortex. Sulforhodamine 101 was injected intraperitoneally to mice to fluorescently label blood plasma[1]. Cortical surface and parenchymal arteries at depths of 100, 200, 300 and 400 µm were scanned with either confocal or two-photon laser scanning microscopy (TCS SP5; Leica), and the arterial response to whisker stimulation (10Hz for 5 sec) was observed. Laser-Doppler flowmetry (FLO-C1; OMEGA FLO) was used to evaluate evoked CBF around the vascular imaging site. Results and Discussion: We observed that the CBF induced by whisker stimulation increased by 15 +/- 6% (N = 4). Mean arterial diameters during the resting state were 39 +/- 7 mum (N = 4), 19 +/- 1 mum (N = 4), 16 +/- 4 mum (N = 7), 17 +/- 4 mum (N = 9), and 14 +/- 3 mum (N = 9) at depths of 0, 100, 200, 300, and 400 mum, respectively. In response to whisker stimulation, the arterial diameter increased significantly (P < 0.01) by 8.8%, 9.9%, 10.9%, 9.2%, and 10.3% relative to the resting state diameters at depths of 0, 100, 200, 300, and 400 mum, respectively. No significant differences were observed across the cortical surface and parenchymal tissue (P = 0.29). In conclusion, we found that the arteries on the cortical surface and in parenchymal tissue within depths of 100 to 400 µm similarly dilated during sensory stimulation in awake mouse brain. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | ISOTT2012 | |||||
| 発表年月日 | ||||||
| 日付 | 2012-08-23 | |||||
| 日付タイプ | Issued | |||||
