@misc{oai:repo.qst.go.jp:00064780,
 author = {Sekiguchi, Yuta and Masamoto, Kazuto and Takuwa, Hiroyuki and Kawaguchi, Hiroshi and Kanno, Iwao and Ito, Hiroshi and Tomita, Yutaka and Suzuki, Norihiro and Sudo, Ryo and Tanishita, Kazuo and 関口 優太 and 正本 和人 and 田桑 弘之 and 川口 拓之 and 菅野 巖 and 伊藤 浩 and 冨田 裕},
 month = {Aug},
 note = {Objectives: Neural activity-induced responses of the arteries on the cortical surface and parenchymal tissue have a critical role in balancing oxygen supply and demand in the brain. However, the relationship between changes to arterial resistance (i.e., vasodilation) and the resultant increases of cerebral blood flow (CBF) remains incompletely understood. In the present study, we measured changes in the diameter of arteries on the cortical surface and in parenchymal tissue induced by sensory stimulation in awake mice. 
Methods: STOCK Tg[Tie2GFP] 287Sato/J mice (N = 7), in which the vascular endothelium had been genetically engineered to express green fluorescent protein, were used for the experiments with a chronic cranial window over the somatosensory cortex.  Sulforhodamine 101 was injected intraperitoneally to mice to fluorescently label blood plasma[1]. Cortical surface and parenchymal arteries at depths of 100, 200, 300 and 400 µm were scanned with either confocal or two-photon laser scanning microscopy (TCS SP5; Leica), and the arterial response to whisker stimulation (10Hz for 5 sec) was observed. Laser-Doppler flowmetry (FLO-C1; OMEGA FLO) was used to evaluate evoked CBF around the vascular imaging site. 
Results and Discussion: We observed that the CBF induced by whisker stimulation increased by 15 +/- 6% (N = 4). Mean arterial diameters during the resting state were 39 +/- 7 mum (N = 4), 19 +/- 1 mum (N = 4), 16 +/- 4 mum (N = 7), 17 +/- 4 mum (N = 9), and 14 +/- 3 mum (N = 9) at depths of 0, 100, 200, 300, and 400 mum, respectively. In response to whisker stimulation, the arterial diameter increased significantly (P < 0.01) by 8.8%, 9.9%, 10.9%, 9.2%, and 10.3% relative to the resting state diameters at depths of 0, 100, 200, 300, and 400 mum, respectively. No significant differences were observed across the cortical surface and parenchymal tissue (P = 0.29). In conclusion, we found that the arteries on the cortical surface and in parenchymal tissue within depths of 100 to 400 &micro;m similarly dilated during sensory stimulation in awake mouse brain., ISOTT2012},
 title = {Measuring the vascular diameter of brain surface arteries and parenchymal arterioles in awake mouse},
 year = {2012}
}