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Effects of Carbon Ion Beam on Pancreatic Cancer Stem-Like Cells In Vitro and In Vivo
https://repo.qst.go.jp/records/64601
https://repo.qst.go.jp/records/646019b03c7a9-fe23-4794-94a9-50619bb522d8
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-06-01 | |||||
| タイトル | ||||||
| タイトル | Effects of Carbon Ion Beam on Pancreatic Cancer Stem-Like Cells In Vitro and In Vivo | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Sai, Sei
× Sai, Sei× Oonishi, Kazuhiko× Yamada, Shigeru× Kamada, Tadashi× 崔 星× 大西 和彦× 山田 滋× 鎌田 正 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Purpose To investigate whether a carbon ion beam has beneficial effects compared to X-rays by targeting putative human pancreatic cancer stem cells. Materials Human pancreatic cancer stem-like cells sorted from MiaPaca2, BxPc3, PANC1 and PK45 cells were treated with or without carbon ion or X-ray irradiation and then colony formation assay, spheroid formation assay, FACS analysis, as well as tumor formation analysis were performed. Results The relative biological effectiveness (RBE) values of a carbon ion beam relative to X-ray for above pancreatic cancer cells at the D10 values were 1.71-2.18. The ability for colony, spheroid formation, and tumorigenicity from cancer stem-like CD44+/CD24+ cells is significantly higher than that from non-cancer stem-like cells CD44-/CD24-cells. FACS data showed that CD44+/CD24+ cells were more highly enriched after X-rays compared to carbon ion irradiation at isoeffective doses. The RBE values for the carbon ion beam relative to X-ray at the D10 levels for CD44+/CD24+ cells were 2.0-2.19. The number of gammaH2AX foci in CD44-/CD24- cells was higher than that of CD44+/CD24+ cells after irradiation with either X-ray or carbon ion beam. The number of gammaH2AX foci in CD44+/CD24+ cells was almost the same in the early time, but it persists for significantly longer in carbon ion beam irradiated cells compared to X-rays. Conclusion Carbon ion beam has superior potential to kill pancreatic cancer stem cell-like cells, and prolonged induction of DNA damage might be one of the pivotal mechanisms of its high radiobiological effects compared to X-rays. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 51st Annual Meeting of Particle Therapy Co-Operative Group (PTCOG51) | |||||
| 発表年月日 | ||||||
| 日付 | 2012-05-19 | |||||
| 日付タイプ | Issued | |||||
