{"created":"2023-05-15T14:47:06.430545+00:00","id":64601,"links":{},"metadata":{"_buckets":{"deposit":"77c6a424-d5c5-4cd2-915e-a405c0076590"},"_deposit":{"created_by":1,"id":"64601","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"64601"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00064601","sets":["10:29"]},"author_link":["636633","636637","636638","636635","636639","636634","636636","636632"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2012-05-19","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Purpose\nTo investigate whether a carbon ion beam has beneficial effects compared to X-rays by targeting putative human pancreatic cancer stem cells. \nMaterials\nHuman pancreatic cancer stem-like cells sorted from MiaPaca2, BxPc3, PANC1 and PK45 cells were treated with or without carbon ion or X-ray irradiation and then colony formation assay, spheroid formation assay, FACS analysis, as well as tumor formation analysis were performed.  \nResults\nThe relative biological effectiveness (RBE) values of a carbon ion beam relative to X-ray for above pancreatic cancer cells at the D10 values were 1.71-2.18. The ability for colony, spheroid formation, and tumorigenicity from cancer stem-like CD44+/CD24+ cells is significantly higher than that from non-cancer stem-like cells CD44-/CD24-cells. FACS data showed that CD44+/CD24+ cells were more highly enriched after X-rays compared to carbon ion irradiation at isoeffective doses. The RBE values for the carbon ion beam relative to X-ray at the D10 levels for CD44+/CD24+ cells were 2.0-2.19. The number of gammaH2AX foci in CD44-/CD24- cells was higher than that of CD44+/CD24+ cells after irradiation with either X-ray or carbon ion beam. The number of gammaH2AX foci in CD44+/CD24+ cells was almost the same in the early time, but it persists for significantly longer in carbon ion beam irradiated cells compared to X-rays. \nConclusion\nCarbon ion beam has superior potential to kill pancreatic cancer stem cell-like cells, and prolonged induction of DNA damage might be one of the pivotal mechanisms of its high radiobiological effects compared to X-rays.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"51st Annual Meeting of Particle Therapy Co-Operative Group (PTCOG51)","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sai, Sei"}],"nameIdentifiers":[{"nameIdentifier":"636632","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Oonishi, Kazuhiko"}],"nameIdentifiers":[{"nameIdentifier":"636633","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamada, Shigeru"}],"nameIdentifiers":[{"nameIdentifier":"636634","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kamada, Tadashi"}],"nameIdentifiers":[{"nameIdentifier":"636635","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"崔 星","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"636636","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"大西 和彦","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"636637","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"山田 滋","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"636638","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鎌田 正","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"636639","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Effects of Carbon Ion Beam on Pancreatic Cancer Stem-Like Cells In Vitro and In Vivo","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Effects of Carbon Ion Beam on Pancreatic Cancer Stem-Like Cells In Vitro and In Vivo"}]},"item_type_id":"10005","owner":"1","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-06-01"},"publish_date":"2012-06-01","publish_status":"0","recid":"64601","relation_version_is_last":true,"title":["Effects of Carbon Ion Beam on Pancreatic Cancer Stem-Like Cells In Vitro and In Vivo"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T21:09:46.585479+00:00"}