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対数増殖条件下におけるマウスES細胞由来神経幹細胞のX線照射に対する放射線感受性の測定
https://repo.qst.go.jp/records/64340
https://repo.qst.go.jp/records/64340cb42cb21-b328-4a54-a29c-69f309f6b967
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2011-10-06 | |||||
| タイトル | ||||||
| タイトル | 対数増殖条件下におけるマウスES細胞由来神経幹細胞のX線照射に対する放射線感受性の測定 | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
磯野, 真由
× 磯野, 真由× 小西, 輝昭× 大津, 昌弘× 吉江, 拓也× 大森, 啓之× 塩見, 尚子× 酢屋, 徳啓× 小林, 亜利紗× 中山, 孝× 井上, 順雄× 小西 輝昭× 塩見 尚子× 酢屋 徳啓× 小林 亜利紗 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Radiation exposure in a developing fetal period is known to cause neuronal d isorders, such as microcephaly and mental retardation, which are considered mainly due to the radiation damage produced in neural stem cells (NSCs). NSC s, which are the dominant number in the fetal brain, have an ability to prol iferate themselves, and to differentiate into other cell types that construc t the central nervous system (CNS), such as neurons, astrocytes, and oligode ndrocytes. Our aim is to clarify the cellular and molecular responses of NSC s against ionizing radiation. In this presentation, we will focus on the rad iation sensitivity related to cell inactivation of NSCs in growth phase. Fol lowing biological endpoints were measured to demonstrate the radio-sensitivi ty of NSCs: maintenance of their ability to proliferate as NSCs, growth curv e, cell cycle regulation, apoptosis, and DNA double strand break repair. The NSCs, derived from embryonic stem cells (HK) of C57BL/6 mice using Neural S tem Sphere method, were irradiated up to 10 Gy by 200 kVp X-ray. Growth curv e were measured up to 4 days after irradiation. Cell cycle analysis was perf ormed flow cytometry to detect G1 and G2/M arrest. Poly ADP ribose polymeras e (PARP) were used as a marker for apoptosis induction and detected with wes tern blot analysis. Growth delay, cell cycle arrest and cleaved PARP were de tected with 1 Gy irradiation. DNA double strand break and its repair were ob served by immunostaining against phospholyrated H2AX, and fluorescence of th e γ-H2AX decreased after irradiation, indicating its repair. As a conc lusion, 1 Gy irradiation induced growth delay, cell cycle arrest, and apopto sis induction. These results may indicate that NSCs in growth phase are high ly sensitive compared to other well-known somatic cells. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 第54回日本神経化学会大会 | |||||
| 発表年月日 | ||||||
| 日付 | 2011-09-28 | |||||
| 日付タイプ | Issued | |||||
