@misc{oai:repo.qst.go.jp:00064340,
 author = {磯野, 真由 and 小西, 輝昭 and 大津, 昌弘 and 吉江, 拓也 and 大森, 啓之 and 塩見, 尚子 and 酢屋, 徳啓 and 小林, 亜利紗 and 中山, 孝 and 井上, 順雄 and 小西 輝昭 and 塩見 尚子 and 酢屋 徳啓 and 小林 亜利紗},
 month = {Sep},
 note = {Radiation exposure in a developing fetal period is known to cause neuronal d isorders, such as microcephaly and mental retardation, which are considered mainly due to the radiation damage produced in neural stem cells (NSCs). NSC s, which are the dominant number in the fetal brain, have an ability to prol iferate themselves, and to differentiate into other cell types that construc t the central nervous system (CNS), such as neurons, astrocytes, and oligode ndrocytes. Our aim is to clarify the cellular and molecular responses of NSC s against ionizing radiation. In this presentation, we will focus on the rad iation sensitivity related to cell inactivation of NSCs in growth phase. Fol lowing biological endpoints were measured to demonstrate the radio-sensitivi ty of NSCs: maintenance of their ability to proliferate as NSCs, growth curv e, cell cycle regulation, apoptosis, and DNA double strand break repair. The  NSCs, derived from embryonic stem cells (HK) of C57BL/6 mice using Neural S tem Sphere method, were irradiated up to 10 Gy by 200 kVp X-ray. Growth curv e were measured up to 4 days after irradiation. Cell cycle analysis was perf ormed flow cytometry to detect G1 and G2/M arrest. Poly ADP ribose polymeras e (PARP) were used as a marker for apoptosis induction and detected with wes tern blot analysis. Growth delay, cell cycle arrest and cleaved PARP were de tected with 1 Gy irradiation. DNA double strand break and its repair were ob served by immunostaining against phospholyrated H2AX, and fluorescence of th e γ-H2AX decreased after irradiation, indicating its repair. As a conc lusion, 1 Gy irradiation induced growth delay, cell cycle arrest, and apopto sis induction. These results may indicate that NSCs in growth phase are high ly sensitive compared to other well-known somatic cells., 第54回日本神経化学会大会},
 title = {対数増殖条件下におけるマウスES細胞由来神経幹細胞のX線照射に対する放射線感受性の測定},
 year = {2011}
}