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Radio-adaptive response of hprt mutation in normal human fibroblasts induced by proton microbeams.
https://repo.qst.go.jp/records/64048
https://repo.qst.go.jp/records/640482d1606b0-a70b-4dad-809b-924fc575b015
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2010-10-04 | |||||
| タイトル | ||||||
| タイトル | Radio-adaptive response of hprt mutation in normal human fibroblasts induced by proton microbeams. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Suzuki, Masao
× Suzuki, Masao× Tsuruoka, Chizuru× Konishi, Teruaki× Oikawa, Masakazu× Hua, Liu Cui× Kaneko, Yumiko× Furusawa, Yoshiya× 鈴木 雅雄× 鶴岡 千鶴× 小西 輝昭× 及川 将一× 劉 翠華× 金子 由美子× 古澤 佳也 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Recently we have investigated that the mutation frequency at the hypoxanthine-guanine phosphoribosyltransferase (hprt) locus, which was detected with measuring 6-thioguanine resistant clones, in normal human fibroblasts induced by the 200kVp X-ray challenging dose (1.5Gy) was reduced at 0.15 times in cells pre-treated with low-dose-rate neutrons (1mSv/8h) as a priming dose compared to unpre-treated cells. Furthermore, the reduced mutation frequency was returned to the control level, when using a specific inhibitor of gap-junction mediated cell-cell communication (40µM lindane). We set up a hypothesis that recoiled protons emitted by the interaction between primary neutrons and surroundings near irradiated cells induce radio-adaptive response in irradiated cell population via gap-junction mediated bystander effect. To examine the hypothesis around 1.5% of total cells were irradiated with single 3.4MeV proton before irradiating the X-ray challenging dose using the microbeam irradiation system, Single Particle Irradiation system to Cell (SPICE) in National Institute of Radiological Sciences, Japan. The result clearly showed that the X-ray induced mutation frequency of hprt locus was suppressed in cells pre-treated with proton microbeams and returned to the control level, when using a specific inhibitor of gap-junction mediated cell-cell communication. The result suggests that neutron-induced adaptive response is caused by recoiled protons and gap-junction mediated bystander effect plays an important role to induce such cellular response. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 56th Annual Meeting of Radiation Research Society | |||||
| 発表年月日 | ||||||
| 日付 | 2010-09-29 | |||||
| 日付タイプ | Issued | |||||
