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重粒子線のマウスES細胞の発生に対する影響
https://repo.qst.go.jp/records/63776
https://repo.qst.go.jp/records/63776b92616bd-6359-41c3-869e-fcbb0e60d743
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2010-02-05 | |||||
| タイトル | ||||||
| タイトル | 重粒子線のマウスES細胞の発生に対する影響 | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
森田, 隆
× 森田, 隆× 吉田, 佳世× 吉田, 周平× 笠井, 清美× 森田 隆× 吉田 佳世× 吉田 周平× 笠井 清美 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Now the manned long term stay in space is realized, the problem that becomes top priority is effects of space radiation on the human body. Especially, exposure of radiation at a low dose and long period is a serious to human germ cells, because the damage will transmit to our descendant. The best way to examine the space radiation effect is to launch laboratory animal in space. However, it is difficult to launch and to breed the mammals in space. So, we come to think usage of the mouse embryonic stem cell (ES cell) as alternative. The freezing mouse ES cell will be preserved in the freezer (MELFI) of kibou in ISS for a long time. After retrieving the frozen cells to the ground, we microinject the ES cells to normal eight cell embryos. Then, we can examine the early development of the embryos in vitro to the blastocyst stage. We irradiated the mouse ES cells with the iron ion beam (500 MeV/u) by using the accelerator HIMAC in Chiba, and microinjected the cells to embryos. During their culture in vitro, we found that the mouse early development was impaired depending on the dose of Fe ion irradiation. In unirradiated condition, more than 70% of the injected embryos developed to normal blastocyst, but after 5 Gy of Fe ion irradiation, 42% of them became blastocyst. Some of the embryos arrested at the morula stage and some were degenerated. It indicated that our method was effective to analyze the space radiation effect on the early development and applicable to biological evaluation of influence on human body. * This research was supported by Ground-based Research Program for Space Utilization promoted by Japan Space Forum and was carried out as collaborative research project with Heavy Ions at NIRS-HIMAC. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 日本宇宙生物科学会第２３回大会 | |||||
| 発表年月日 | ||||||
| 日付 | 2009-10-03 | |||||
| 日付タイプ | Issued | |||||
