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Biological studies using proton microbeam irradiation system, SPICE
https://repo.qst.go.jp/records/63670
https://repo.qst.go.jp/records/63670a1e8da04-4209-4044-b729-60f016b442c1
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2009-11-03 | |||||
| タイトル | ||||||
| タイトル | Biological studies using proton microbeam irradiation system, SPICE | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Konishi, Teruaki
× Konishi, Teruaki× Oikawa, Masakazu× Iso, Hiroyuki× Ishikawa, Takahiro× Higuchi, Yuichi× Kodama, Kumiko× Isono, Mayu× Yasuda, Nakahiro× Suya, Noriyoshi× Imaseki, Hitoshi× 小西 輝昭× 及川 将一× 磯 浩之× 石川 剛弘× 樋口 有一× 児玉 久美子× 安田 仲宏× 酢屋 徳啓× 今関 等 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The development of SPICE (Single Particle Irradiation system to Cell), a microbeam irradiation system, has been completed at the National Institute of Radiological Sciences (NIRS). The beam size has been improved to approximately 2micro in diameter, and the cell targeting system can irradiate up to 400 cells per minute. Cell dish has been specially designed: a 2.5 micro Mylar film stretched by pressing with a metal ring. A dish is placed on a voice coil stage equipped on the cell targeting system, which includes a fluorescent microscope and a CCD camera for capturing cell images. This microscope system captures images of dyed cell nuclei, computes the location coordinates of individual cells, and synchronizes this with the voice coil motor stage and single-particle irradiation system consisting of a scintillation counter and beam deflector. Irradiation of selected cells with a programmable number of protons is now automatable. We employed the simultaneous detection method for visualizing the position of mammalian cells and proton traversal through CR-39 to determine whether the targeted cells are actually irradiated. An Immuno-assay was also performed against gamma-H2AX, to confirm the induction of DNA double-strand breaks in the target cells. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 第47回日本生物物理学会年会 | |||||
| 発表年月日 | ||||||
| 日付 | 2009-11-01 | |||||
| 日付タイプ | Issued | |||||
