@misc{oai:repo.qst.go.jp:00063670,
 author = {Konishi, Teruaki and Oikawa, Masakazu and Iso, Hiroyuki and Ishikawa, Takahiro and Higuchi, Yuichi and Kodama, Kumiko and Isono, Mayu and Yasuda, Nakahiro and Suya, Noriyoshi and Imaseki, Hitoshi and 小西 輝昭 and 及川 将一 and 磯 浩之 and 石川 剛弘 and 樋口 有一 and 児玉 久美子 and 安田 仲宏 and 酢屋 徳啓 and 今関 等},
 month = {Nov},
 note = {The development of SPICE (Single Particle Irradiation system to Cell), a microbeam irradiation system, has been completed at the National Institute of Radiological Sciences (NIRS). The beam size has been improved to approximately 2micro in diameter, and the cell targeting system can irradiate up to 400 cells per minute. Cell dish has been specially designed: a 2.5 micro Mylar film stretched by pressing with a metal ring. A dish is placed on a voice coil stage equipped on the cell targeting system, which includes a fluorescent microscope and a CCD camera for capturing cell images. This microscope system captures images of dyed cell nuclei, computes the location coordinates of individual cells, and synchronizes this with the voice coil motor stage and single-particle irradiation system consisting of a scintillation counter and beam deflector. Irradiation of selected cells with a programmable number of protons is now automatable. We employed the simultaneous detection method for visualizing the position of mammalian cells and proton traversal through CR-39 to determine whether the targeted cells are actually irradiated. An Immuno-assay was also performed against gamma-H2AX, to confirm the induction of DNA double-strand breaks in the target cells., 第47回日本生物物理学会年会},
 title = {Biological studies using proton microbeam irradiation system, SPICE},
 year = {2009}
}