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Detection of Leukemia-Specific Mutations in Mice Exposed to Radiation
https://repo.qst.go.jp/records/62955
https://repo.qst.go.jp/records/629558f8a0f78-1234-42c3-ae83-49638c9da25c
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2009-01-31 | |||||
| タイトル | ||||||
| タイトル | Detection of Leukemia-Specific Mutations in Mice Exposed to Radiation | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
伴, 信彦
× 伴, 信彦× 柿沼, 志津子× 大町, 康× 甲斐, 倫明× 伴 信彦× 柿沼 志津子× 大町 康× 甲斐 倫明 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | X-ray induces myeloid leukemia in C3H/He mice with the incidence of 20-25% at 3Gy. Mutation of Sfpi1 (PU.1) is crucial in the development of myeloid leukemia. The mutation, however, is mostly specific point mutations in the DNA-binding Ets domain of PU.1, and they are unlikely to be a direct consequence of radiation-induced DNA damage. To elucidate when and how often this type of mutation arises in hematopoietic cells, we have developed a technique that is capable of detecting a small number of mutated cells in the vast majority of non-mutated cells. The technique is based on the wild-type blocking PCR, in which LNA (locked nucleic acid) oligonucleotide blocks amplification of wild-type DNA during PCR while permitting amplification of mutants. The PCR followed by restriction enzyme digestion has enabled to detect mutant DNA in 104 times amount of wild-type DNA. This technique is being applied to DNA samples from C3H/He mice that were exposed to 3Gy of x-rays and were maintained for more than a year. Among 12 exposed mice and 6 age-matched controls analyzed so far, the mutation has been detected in a sample from the spleen of an exposed mouse. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 第５１回日本放射線影響学会 | |||||
| 発表年月日 | ||||||
| 日付 | 2008-11-21 | |||||
| 日付タイプ | Issued | |||||
