{"created":"2023-05-15T14:46:05.205302+00:00","id":62955,"links":{},"metadata":{"_buckets":{"deposit":"e06b0b9c-6a62-4bef-af74-e3c4991c38a0"},"_deposit":{"created_by":1,"id":"62955","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"62955"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00062955","sets":["10:29"]},"author_link":["621896","621898","621903","621900","621899","621902","621901","621897"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2008-11-21","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"X-ray induces myeloid leukemia in C3H/He mice with the incidence of 20-25% at 3Gy. Mutation of Sfpi1 (PU.1) is crucial in the development of myeloid leukemia. The mutation, however, is mostly specific point mutations in the DNA-binding Ets domain of PU.1, and they are unlikely to be a direct consequence of radiation-induced DNA damage. To elucidate when and how often this type of mutation arises in hematopoietic cells, we have developed a technique that is capable of detecting a small number of mutated cells in the vast majority of non-mutated cells. The technique is based on the wild-type blocking PCR, in which LNA (locked nucleic acid) oligonucleotide blocks amplification of wild-type DNA during PCR while permitting amplification of mutants. The PCR followed by restriction enzyme digestion has enabled to detect mutant DNA in 104 times amount of wild-type DNA. This technique is being applied to DNA samples from C3H/He mice that were exposed to 3Gy of x-rays and were maintained for more than a year. Among 12 exposed mice and 6 age-matched controls analyzed so far, the mutation has been detected in a sample from the spleen of an exposed mouse.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第５１回日本放射線影響学会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"伴, 信彦"}],"nameIdentifiers":[{"nameIdentifier":"621896","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"柿沼, 志津子"}],"nameIdentifiers":[{"nameIdentifier":"621897","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"大町, 康"}],"nameIdentifiers":[{"nameIdentifier":"621898","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"甲斐, 倫明"}],"nameIdentifiers":[{"nameIdentifier":"621899","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"伴 信彦","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"621900","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"柿沼 志津子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"621901","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"大町 康","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"621902","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"甲斐 倫明","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"621903","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Detection of Leukemia-Specific Mutations in Mice Exposed to Radiation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Detection of Leukemia-Specific Mutations in Mice Exposed to Radiation"}]},"item_type_id":"10005","owner":"1","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-01-31"},"publish_date":"2009-01-31","publish_status":"0","recid":"62955","relation_version_is_last":true,"title":["Detection of Leukemia-Specific Mutations in Mice Exposed to Radiation"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T21:24:50.217427+00:00"}