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Experimental determination of individual patient's haplotypes of radiation sensitivity-associated gene
https://repo.qst.go.jp/records/62677
https://repo.qst.go.jp/records/6267797fa35d1-309f-4679-82d7-5af9d434fcbc
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2008-08-15 | |||||
| タイトル | ||||||
| タイトル | Experimental determination of individual patient's haplotypes of radiation sensitivity-associated gene | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Michikawa, Yuichi
× Michikawa, Yuichi× Iwakawa, Mayumi× Imai, Takashi× 道川 祐市× 岩川 眞由美× 今井 高志 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | A novel methodology of haplotype determination involving sensitive amplification of single chromosomes was developed. Limited dilution of chromosomes was performed within heated alkaline agarose solution to avoid extensive aggregation during dilution process. Aliquoted and solidified agarose gel pieces were then treated by exogenously provided Phi29 DNA polymerase and random hexamer oligonucleotides, yielding up to 120 000-fold amplification of immobilized chromosomes. The amplified materials were recovered in solution in PCR-ready form by simple heating to melt the gel, making them conveniently available for further applications such as multiple locus genotyping. With this amplification methodology, haplotypes of radiation sensitivity-associated gene were successfully evaluated in individual cancer patients. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The 7th International Workshop on Advanced Genomics | |||||
| 発表年月日 | ||||||
| 日付 | 2007-11-28 | |||||
| 日付タイプ | Issued | |||||
