@misc{oai:repo.qst.go.jp:00062677,
 author = {Michikawa, Yuichi and Iwakawa, Mayumi and Imai, Takashi and 道川 祐市 and 岩川 眞由美 and 今井 高志},
 month = {Nov},
 note = {A novel methodology of haplotype determination involving sensitive amplification of single chromosomes was developed. Limited dilution of chromosomes was performed within heated alkaline agarose solution to avoid extensive aggregation during dilution process. Aliquoted and solidified agarose gel pieces were then treated by exogenously provided Phi29 DNA polymerase and random hexamer oligonucleotides, yielding up to 120 000-fold amplification of immobilized chromosomes. The amplified materials were recovered in solution in PCR-ready form by simple heating to melt the gel, making them conveniently available for further applications such as multiple locus genotyping. With this amplification methodology, haplotypes of radiation sensitivity-associated gene were successfully evaluated in individual cancer patients., The 7th International Workshop on Advanced Genomics},
 title = {Experimental determination of individual patient's haplotypes of radiation sensitivity-associated gene},
 year = {2007}
}