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アイテム
Analysisi of genomic organization, developmental expression and cis-regulatory regions of globin genes in medaka Oryzias latipes.
https://repo.qst.go.jp/records/60743
https://repo.qst.go.jp/records/60743bad2ce58-1797-4dab-8055-0d8a0d3fa9e0
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2004-12-14 | |||||
| タイトル | ||||||
| タイトル | Analysisi of genomic organization, developmental expression and cis-regulatory regions of globin genes in medaka Oryzias latipes. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Maruyama, Kouichi
× Maruyama, Kouichi× Yasumasu, Shigeki× Iuchi, Ichiro× Ishikawa, Yuuji× 丸山 耕一× 安増 茂樹× 石川 裕二 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Hemoglobin in vertebrate erythrocytes consists of 2 α-and β-globins. It is well known that globins are switched from embryonic to adult type during development. The mechanism underlying the switching is not sufficiently understood, especially in teleost such as medaka. We determined the full-length structure of globin locus in the medaka. Two loci were identified: One locus consisted of 9 embryonic globin genes and mapped to linkage group (LG) 8, the other comprised 3 adult globin genes and mapped to LG 19. In the loci, 4 pairs of α and β genes were organized in head-to-head structure. Such genomic organization of globin genes of medaka differed from those reported in higher vertebrates such as mammals, chicken and an amphibian. The developmental expression paterns of 11 genes were classified into 4 types: 1) Embryo-specific expression, 2) Expression from embryo to young fish, 3) Expression from young fish to fully-grown adult, 4)Continuous expression from embryo to adult. In the 1-2Kb regions 5'-upstream to respective genes, hematopoietic transcription factor binding sites such as GATA-boxes were found. We fused each of these regions including probable minimum promoters with a reporter gene, introduced the fusion gene into embryos and established 4 types of medaka lines that stably express the reporter gene in erythroid cells. Each transgenic medaka expressed reporter genes in developmental stage specific manner of its downstream genes. Compared with locus control regions in mammals, in medaka a relatively shorter 5'-upstream cis-regulatory region of globin genes had the ability to promote or enhance the erythroid cell- and developmental stage-specific expression. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 第２７回日本分子生物学会年会 | |||||
| 発表年月日 | ||||||
| 日付 | 2004-12-11 | |||||
| 日付タイプ | Issued | |||||
