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Early detection of p53-/- thymocytes appeared during radiation-induced thymic lymphomagenesis in p53 heterozygous (+/-) B10 mice
https://repo.qst.go.jp/records/59901
https://repo.qst.go.jp/records/59901b7e6a6b0-8ab5-4281-8a0a-56dbb466e531
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2003-08-25 | |||||
| タイトル | ||||||
| タイトル | Early detection of p53-/- thymocytes appeared during radiation-induced thymic lymphomagenesis in p53 heterozygous (+/-) B10 mice | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Aizawa, Shirou
× Aizawa, Shirou× Tanaka, Kaoru× Mori, Masahiko× Yoshida, Kazuko× 相澤 志郎× 田中 薫× 森 雅彦× 吉田 和子 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Radiation exposure is know to be highly leukemmogenic, but the mechanism action is not yet understood. Studies with the p53 heterozygous mouse have shown that a single dose of radiation can induce a high incidence of thymic lymphomas, most of which manifest the loss of wild-type p53 allele. The loss of heterozygosity (LOH) in the p53 gene is thus thought to be an early event in radiation-induced thymic lymphomagenesis. Thus, the p53 heterozygous mice might be a useful model for the study of consequences of radiation-exposure. However, sensitive detection of p53-/- thymocytes appeared in p53+/- thymocyte population was needed to examine appearance of p53-/- thymocyte in p53 heterozygous (+/-) B10 mice in the early stage of radiation-induced thymic lymphomagenesis. In this study, we characterized highly radioresistant thymocytes as a candidate of p53-/- thymocytes, because the enrichment of p53-/- thymocyte by a high dose of irradiation could be expected from the facts that p53+/- thymocytes are sensitive to radiation-induced apoptosis but p53-/- thymocytes highly resistant. At various times post irradiation (6Gy) as lymphoma-inducing treatment, p53 heterozygous B10 mice were treated with a high dose (30Gy)of whole-body irradiation to enrich p53-/- thymocytes and, 24 hours later, the remaining thymocytes were assayed for surface marker and p53 genotype. In a significant fraction of the mice 7wks after 6Gy-irradiation, we observe an abnormal increase of relative cell number of CD4+CD8+ thymocytes remaining in the thymus of highly irradiated mice and such abnormal thymocytes could be detected even 5 weeks after the irradiation. The abnormal CD4+CD8+ thymocytes werer shown by PCR analysis of sorted cells to contain cells ith p53-/- genotype. The data obtained so far suggest that p53-/- thymocytes may appear as prelymphoma cells within several weeks post irradiation in CD4+CD8+ thymocyte population of irradiated p53 heterozygous mice. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 第１２回国際放射線研究会議 | |||||
| 発表年月日 | ||||||
| 日付 | 2003-08-22 | |||||
| 日付タイプ | Issued | |||||
