{"created":"2023-05-15T14:43:47.199054+00:00","id":59901,"links":{},"metadata":{"_buckets":{"deposit":"02fd49c2-770a-403b-92b1-cdfc406b2ab6"},"_deposit":{"created_by":1,"id":"59901","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"59901"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00059901","sets":["10:29"]},"author_link":["595890","595896","595892","595893","595891","595897","595895","595894"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2003-08-22","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Radiation exposure is know to be highly leukemmogenic, but the mechanism action is not yet understood. Studies with the p53 heterozygous mouse have shown that a single dose of radiation can induce a high incidence of thymic lymphomas, most of which manifest the loss of wild-type p53 allele.  The loss of heterozygosity (LOH) in the p53 gene is thus thought to be an early event in radiation-induced thymic lymphomagenesis.   Thus, the p53 heterozygous mice might be a useful model for the study of consequences of radiation-exposure.  However, sensitive detection of p53-/- thymocytes appeared in p53+/- thymocyte population was needed to examine appearance of p53-/- thymocyte in p53 heterozygous (+/-) B10 mice in the early stage of radiation-induced thymic lymphomagenesis.  In this study, we characterized highly radioresistant thymocytes as a candidate of p53-/- thymocytes, because the enrichment of p53-/- thymocyte by a high dose of irradiation could be expected from the facts that p53+/- thymocytes are sensitive to radiation-induced apoptosis but p53-/- thymocytes highly resistant.   At various times post irradiation (6Gy) as lymphoma-inducing treatment,  p53 heterozygous B10 mice were treated with a high dose (30Gy)of whole-body irradiation to enrich p53-/- thymocytes and, 24 hours later, the remaining thymocytes were assayed for surface marker and p53 genotype.  In a significant fraction of the mice 7wks after 6Gy-irradiation, we observe an abnormal increase of relative cell number of CD4+CD8+ thymocytes remaining in the thymus of highly irradiated mice and such abnormal thymocytes could be detected even 5 weeks after  the irradiation.   The abnormal CD4+CD8+ thymocytes werer shown by PCR analysis of sorted cells to contain cells ith p53-/- genotype.  The data obtained so far suggest that p53-/- thymocytes may appear as prelymphoma cells within several weeks post irradiation in CD4+CD8+ thymocyte population of irradiated p53 heterozygous mice.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第１２回国際放射線研究会議","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Aizawa, Shirou"}],"nameIdentifiers":[{"nameIdentifier":"595890","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tanaka, Kaoru"}],"nameIdentifiers":[{"nameIdentifier":"595891","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mori, Masahiko"}],"nameIdentifiers":[{"nameIdentifier":"595892","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yoshida, Kazuko"}],"nameIdentifiers":[{"nameIdentifier":"595893","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"相澤 志郎","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"595894","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"田中 薫","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"595895","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"森 雅彦","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"595896","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"吉田 和子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"595897","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Early detection of p53-/- thymocytes appeared during radiation-induced thymic lymphomagenesis in p53 heterozygous (+/-) B10 mice","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Early detection of p53-/- thymocytes appeared during radiation-induced thymic lymphomagenesis in p53 heterozygous (+/-) B10 mice"}]},"item_type_id":"10005","owner":"1","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2003-08-25"},"publish_date":"2003-08-25","publish_status":"0","recid":"59901","relation_version_is_last":true,"title":["Early detection of p53-/- thymocytes appeared during radiation-induced thymic lymphomagenesis in p53 heterozygous (+/-) B10 mice"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T21:59:45.989063+00:00"}