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重粒子線治療における肺癌腫瘍の至適分割法の研究：低酸素下加速分裂細胞の放射線感受性と
https://repo.qst.go.jp/records/55489
https://repo.qst.go.jp/records/55489b609652f-3cb3-4e0f-ba0b-cd1f51f54103
| Item type | 一般雑誌記事 / Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2006-06-23 | |||||
| タイトル | ||||||
| タイトル | 重粒子線治療における肺癌腫瘍の至適分割法の研究：低酸素下加速分裂細胞の放射線感受性と | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
三枝, 公美子
× 三枝, 公美子× 矢代, 智康× 宮本, 祐介× 今井, 高志× 宮本, 忠昭× 藤澤, 武彦× 三枝 公美子× 矢代 智康× 宮本 祐介× 今井 高志× 宮本 忠昭× 藤澤 武彦 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Using cultured and nude mouse tumor cells (IA) derived from a human lung cancer, we had previously reported the early and rapid survival recovery (PLD repair) occurring after irradiation. There are only a few studies in the molecular biological level and gene expression in association with PLD repair, and on limiting its effect after carbon-ion beam irradiation to cancer cells in vivo, no study was reported. In this study we tried to detect the mechanism of DNA damage and repair of the IA tumor cell after carbon-ion beam or X-ray irradiation. At first, colony assay method was performed to compare the time dependent cell survival of the IA cells after each irradiation. Second, to search the genes causing PLD repair after each irradiation, we evaluated the time dependent gene expression by using semi-quantitative RT-PCR with the selected 33 genes reported to be related to DNA repair. From the result of survival assay, PLD repair was found only after irradiation of X-ray in this IA cells, whereas it was inhibited after carbon-ion beam irradiation. Genes showing significantly higher expressions after X-ray irradiation than those after carbon-ion beam irradiation were 5 genes and lower expressions after X-ray irradiation than those after carbon-ion beam irradiation were 7 genes. We think these 12 genes are the candidate ones associated with PLD repair among the selected 33 DNA repair related genes. | |||||
| 書誌情報 |
放射線医学総合研究所重粒子線がん治療装置等共同利用研究報告書 p. 42-43, 発行日 2006-04 |
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| 出版者 | ||||||
| 出版者 | 放医研 | |||||
