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ヒト培養細胞を用いた重粒子線照射における遺伝子発現変化の検討
https://repo.qst.go.jp/records/55322
https://repo.qst.go.jp/records/5532208cfa449-36e2-4789-ad35-b0f38d424a3e
| Item type | 一般雑誌記事 / Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2004-10-06 | |||||
| タイトル | ||||||
| タイトル | ヒト培養細胞を用いた重粒子線照射における遺伝子発現変化の検討 | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
古野, 亜紀
× 古野, 亜紀× 三枝, 公美子× 大塚, 好美× 野島, 久美恵× 今井, 高志× 古野 亜紀× 三枝 公美子× 荘司 好美× 野島 久美恵× 今井 高志 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Understanding the individuals variation in radiation sensitivity at a molecular level may be essential to predict unusual response of normal tissue cells to clinical radiations. Micro array technology has been proved to be a powerful tool for comparing gene expression in normal and disease states and/or for analyzing the response of cells exposed to drugs or unknown physiological conditions. We have studied gene expression profiles of the cultured neonatal human dermal fibroblasts (NHDF) using the custom-made oligonucleotide microarray, containing 22,500 probes (representing 19,862unique genes), before or/after exposure to 0.1, 2 and 5Gy of X rays and carbon-ion beams. Total RNA was purified from the cells at 1, 3, 6, 12, 18, 24 hours after irradiations. The expression level of some genes was confirmed with a quantative reverse transcription-PCR. | |||||
| 書誌情報 |
放射線医学総合研究所重粒子線がん治療装置等共同利用研究報告書 p. 139-140, 発行日 2004-05 |
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| 出版者 | ||||||
| 出版者 | 放医研 | |||||
