WEKO3
アイテム
Genome stability and transcription by RNA polymerase II : Temperature-sensitive mutants of the largest subunit of pol II showing genome instability
https://repo.qst.go.jp/records/53729
https://repo.qst.go.jp/records/5372922b65f37-5118-4739-a020-892a264a0f6f
| Item type | 会議発表論文 / Conference Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2006-06-16 | |||||
| タイトル | ||||||
| タイトル | Genome stability and transcription by RNA polymerase II : Temperature-sensitive mutants of the largest subunit of pol II showing genome instability | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_5794 | |||||
| 資源タイプ | conference paper | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Sugaya, Kimihiko
× Sugaya, Kimihiko× Sasanuma, Shunichi× Ajimura, Masahiro× Tsuji, Hideo× Morimyo, Mitsuoki× Cook, Peter× Mita, Kazuei× 菅谷 公彦× 笹沼 俊一× 味村 正博× 辻 秀雄× 森明 充興× 三田 和英 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | RNA polymerase II (pol II) is a multi-subunit enzyme responsible for the transcription of most genes in eukaryotes. If forms enormous complexes, or holoenzymes, that associates with other proteins involved in related functions like splicing, polyadenylation, and the repair of damage in DNA. In turn, several such holoenzymes are organized into even larger structures called transcription factories (Cook, 1999). At the heart of each individual polymerizing complex lies the catalytic subunit, RPB1 this is the largest subunit found in the core enzyme. Sister chromatid exchange (SCE) is as an indicator of mutagenicity and carcinogenicity. A temperature-sensitive (ts) mutant of the CHO-K1 line, tsTM4, exhibited abnormal induction of SCEs along with a decrease of DNA synthesis in the cells arrested in S phase at the non-permissive temperature. These ts defects of tsTM4 were complemented by the human genomic fragments containing the entire coding region of RPB1 gene, as well as green fluorescent protein (GFP) tagged human rpb1 cDNA. |
|||||
| 書誌情報 |
Proceedings of the Japan-France Workshop on Radiobiology and Isotopic Imaging 巻 4, p. 41-44, 発行日 2002-06 |
|||||
