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Different effects of carbon ion beams and X-rays on clonogenic survival and DNA Repair in human pancreatic cancer stem-like cells
https://repo.qst.go.jp/records/49575
https://repo.qst.go.jp/records/49575edadb7ec-62aa-4365-8720-e9a0b3eacd77
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-12-13 | |||||
| タイトル | ||||||
| タイトル | Different effects of carbon ion beams and X-rays on clonogenic survival and DNA Repair in human pancreatic cancer stem-like cells | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Oonishi, Kazuhiko
× Oonishi, Kazuhiko× Cui, Xing× Hirakawa, Hirokazu× Fujimori, Akira× Kamijo, Takehiko× Yamada, Shigeru× Yokosuka, Osamu× Kamada, Tadashi× Tsujii, Hirohiko× Sai, Sei× Hirakawa, Hirokazu× Fujimori, Akira× Kamijo, Takehiko× Yamada, Shigeru× Yokosuka, Osamu× Kamada, Tadashi |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Purpose: The effects of a carbon ion beam and X-rays on human pancreatic cancer stem-like cells were examined from the point of view of clonogenic survival and DNA repair. Materials and methods: Human pancreatic cancer stem-like cells were treated with and without carbon ion and X-ray irradiation, and then colony, spheroid and tumor formation assays as well as rH2AX foci formation assay were performed. Results: The relative biological effectiveness (RBE) values of a carbon ion beam relative to X-ray for the MIA PaCa-2 and BxPc-3 cells at the D10 values were 1.85–2.10. The ability for colony, spheroid formation, and tumorigenicity from cancer stem-like CD44+/CD24+ cells is significantly higher than that from noncancer stem-like CD44-/CD24-cells. FACS data showed that CD44+/CD24+ cells were more highly enriched after X-rays compared to carbon ion irradiation at isoeffective doses. The RBE values for the carbon ion beam relative to X-ray at the D10 levels for CD44+/CD24+ cells were 2.0–2.19.The number of rH2AX foci in CD44-/CD24-cells was higher than that of CD44+/CD24+ cells after irradiation with either X-ray or carbon ion beam. The number of rH2AX foci in CD44+/CD24+ cells was almost the same in the early time, but it persists significantly longer in carbon ion beam irradiated cells compared to X-rays. Conclusions: Carbon ion beam has superior potential to kill pancreatic cancer stem cell-like cells, and prolonged induction of DNA damage might be one of the pivotal mechanisms of its high radiobiological effects compared to X-rays. |
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| 書誌情報 |
Radiotherapy and Oncology 巻 105, 号 2, p. 258-265, 発行日 2012-09 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0167-8140 | |||||
| 関連サイト | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | http://dx.doi.org/10.1016/j.radonc.2012.08.009 | |||||
