{"created":"2023-05-15T14:38:24.503556+00:00","id":49575,"links":{},"metadata":{"_buckets":{"deposit":"7274befd-6434-4759-ab1a-56c15307b64b"},"_deposit":{"created_by":1,"id":"49575","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"49575"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00049575","sets":["1"]},"author_link":["811279","811275","811282","811272","811274","811268","811269","811271","811280","811278","811281","811277","811270","811276","811267","811273"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"265","bibliographicPageStart":"258","bibliographicVolumeNumber":"105","bibliographic_titles":[{"bibliographic_title":"Radiotherapy and Oncology"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Purpose: The effects of a carbon ion beam and X-rays on human pancreatic cancer stem-like cells were examined from the point of view of clonogenic survival and DNA repair.\nMaterials and methods: Human pancreatic cancer stem-like cells were treated with and without carbon ion and X-ray irradiation, and then colony, spheroid and tumor formation assays as well as rH2AX foci formation assay were performed.\n\nResults: The relative biological effectiveness (RBE) values of a carbon ion beam relative to X-ray for the MIA PaCa-2 and BxPc-3 cells at the D10 values were 1.85–2.10. The ability for colony, spheroid formation, and tumorigenicity from cancer stem-like CD44+/CD24+ cells is significantly higher than that from noncancer stem-like CD44-/CD24-cells. FACS data showed that CD44+/CD24+ cells were more highly enriched after X-rays compared to carbon ion irradiation at isoeffective doses. \nThe RBE values for the carbon ion beam relative to X-ray at the D10 levels for CD44+/CD24+ cells were 2.0–2.19.The number of rH2AX foci in CD44-/CD24-cells was higher than that of CD44+/CD24+ cells after irradiation with either X-ray or carbon ion beam. The number of rH2AX foci in CD44+/CD24+ cells was almost the same in the early time, but it persists significantly longer in carbon ion beam irradiated cells compared to X-rays.\nConclusions: Carbon ion beam has superior potential to kill pancreatic cancer stem cell-like cells, and prolonged induction of DNA damage might be one of the pivotal mechanisms of its high radiobiological effects compared to X-rays.","subitem_description_type":"Abstract"}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1016/j.radonc.2012.08.009","subitem_relation_type_select":"DOI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0167-8140","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Oonishi, Kazuhiko"}],"nameIdentifiers":[{"nameIdentifier":"811267","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Cui, Xing"}],"nameIdentifiers":[{"nameIdentifier":"811268","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hirakawa, Hirokazu"}],"nameIdentifiers":[{"nameIdentifier":"811269","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fujimori, Akira"}],"nameIdentifiers":[{"nameIdentifier":"811270","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kamijo, Takehiko"}],"nameIdentifiers":[{"nameIdentifier":"811271","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamada, Shigeru"}],"nameIdentifiers":[{"nameIdentifier":"811272","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yokosuka, Osamu"}],"nameIdentifiers":[{"nameIdentifier":"811273","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kamada, Tadashi"}],"nameIdentifiers":[{"nameIdentifier":"811274","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tsujii, Hirohiko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"811275","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sai, Sei","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"811276","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hirakawa, Hirokazu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"811277","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fujimori, Akira","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"811278","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kamijo, Takehiko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"811279","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamada, Shigeru","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"811280","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yokosuka, Osamu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"811281","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kamada, Tadashi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"811282","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Different effects of carbon ion beams and  X-rays on clonogenic survival and DNA Repair in human pancreatic cancer stem-like cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Different effects of carbon ion beams and  X-rays on clonogenic survival and DNA Repair in human pancreatic cancer stem-like cells"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-12-13"},"publish_date":"2012-12-13","publish_status":"0","recid":"49575","relation_version_is_last":true,"title":["Different effects of carbon ion beams and  X-rays on clonogenic survival and DNA Repair in human pancreatic cancer stem-like cells"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:33:38.595992+00:00"}