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Purification and structural characterization of water-soluble menaquinone-7 produced by Bacillus subtilis natto
https://repo.qst.go.jp/records/49154
https://repo.qst.go.jp/records/4915497e7d579-a326-4099-8f25-a8c4f3b8880e
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2019-02-18 | |||||
| タイトル | ||||||
| タイトル | Purification and structural characterization of water-soluble menaquinone-7 produced by Bacillus subtilis natto | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Chatake, Toshiyuki
× Chatake, Toshiyuki× Yanagisawa, Yasuhide× Inoue, Rintaro× Sugiyama, Masaaki× 松尾, 龍人× 藤原, 悟× Ohsugi, Tadanori× Sumi, Hiroyuki× 松尾 龍人× 藤原 悟 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Vitamin K is essential for osteogenesis, and vitamin K deficiency causes osteoporosis, leading to hip fracture and coronary disease. Menaquinone-7 (MK-7), one of vitamin K subtypes, is abundantly contained in Japanese traditional fermented food natto. Bacillus subtilis natto secretes a water-soluble macromolecular complex consisting of MK-7 and peptides (natto-MK-7), although MK-7 itself is water-insoluble. However, structural properties of natto-MK-7 have not been well elucidated. In the present study, natto-MK-7 was purified from a liquid culture medium of Bacillus subtilis natto by fast protein liquid chromatography. The protein assay and electrophoresis suggest that natto-MK-7 is a complex composed of multi components including amphiphilic compounds. The hydrodynamic radius of natto-MK-7 determined by the dynamic light scattering experiment suggests that natto-MK-7 has a more compact shape than that predicted in the previous size-exclusion chromatography. | |||||
| 書誌情報 |
Journal of Food biochemistry 巻 42, 号 6, 発行日 2018-08 |
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| 出版者 | ||||||
| 出版者 | Wiley | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1111/jfbc.12630 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://onlinelibrary.wiley.com/doi/abs/10.1111/jfbc.12630 | |||||
| 関連名称 | https://onlinelibrary.wiley.com/doi/abs/10.1111/jfbc.12630 | |||||
