{"created":"2023-05-15T14:38:05.913459+00:00","id":49154,"links":{},"metadata":{"_buckets":{"deposit":"20bd81c5-770a-445c-8eab-df14db915139"},"_deposit":{"created_by":1,"id":"49154","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"49154"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00049154","sets":["1"]},"author_link":["723794","723795","723793","723796","723798","723791","723797","723789","723790","723792"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicVolumeNumber":"42","bibliographic_titles":[{"bibliographic_title":"Journal of Food biochemistry"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Vitamin K is essential for osteogenesis, and vitamin K deficiency causes osteoporosis, leading to hip fracture and coronary disease. Menaquinone-7 (MK-7), one of vitamin K subtypes, is abundantly contained in Japanese traditional fermented food natto. Bacillus subtilis natto secretes a water-soluble macromolecular complex consisting of MK-7 and peptides (natto-MK-7), although MK-7 itself is water-insoluble. However, structural properties of natto-MK-7 have not been well elucidated. In the present study, natto-MK-7 was purified from a liquid culture medium of Bacillus subtilis natto by fast protein liquid chromatography. The protein assay and electrophoresis suggest that natto-MK-7 is a complex composed of multi components including amphiphilic compounds. ‎The hydrodynamic radius of natto-MK-7 determined by the dynamic light scattering experiment suggests that natto-MK-7 has a more compact shape than that predicted in the previous size-exclusion chromatography.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Wiley"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1111/jfbc.12630","subitem_relation_type_select":"DOI"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://onlinelibrary.wiley.com/doi/abs/10.1111/jfbc.12630"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://onlinelibrary.wiley.com/doi/abs/10.1111/jfbc.12630","subitem_relation_type_select":"URI"}}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Chatake, Toshiyuki"}],"nameIdentifiers":[{"nameIdentifier":"723789","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yanagisawa, Yasuhide"}],"nameIdentifiers":[{"nameIdentifier":"723790","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Inoue, Rintaro"}],"nameIdentifiers":[{"nameIdentifier":"723791","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sugiyama, Masaaki"}],"nameIdentifiers":[{"nameIdentifier":"723792","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"松尾, 龍人"}],"nameIdentifiers":[{"nameIdentifier":"723793","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"藤原, 悟"}],"nameIdentifiers":[{"nameIdentifier":"723794","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ohsugi, Tadanori"}],"nameIdentifiers":[{"nameIdentifier":"723795","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sumi, Hiroyuki"}],"nameIdentifiers":[{"nameIdentifier":"723796","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"松尾 龍人","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"723797","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"藤原 悟","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"723798","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Purification and structural characterization of water-soluble menaquinone-7 produced by Bacillus subtilis natto","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Purification and structural characterization of water-soluble menaquinone-7 produced by Bacillus subtilis natto"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-02-18"},"publish_date":"2019-02-18","publish_status":"0","recid":"49154","relation_version_is_last":true,"title":["Purification and structural characterization of water-soluble menaquinone-7 produced by Bacillus subtilis natto"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T19:29:38.281049+00:00"}