WEKO3
アイテム
Polypentagonal ice-like water networks emerge solely in an activity-improved variant of ice-binding protein
https://repo.qst.go.jp/records/49084
https://repo.qst.go.jp/records/49084498840fc-a48c-48a5-a9d4-935b52740cf5
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2018-06-12 | |||||
| タイトル | ||||||
| タイトル | Polypentagonal ice-like water networks emerge solely in an activity-improved variant of ice-binding protein | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Mahatabuddin , Sheikh
× Mahatabuddin , Sheikh× Fukami , Daichi× Arai, Tatsuya× Nishimiya, Yoshiyuki× Shimizu, Rumi× Shibazaki , Chie× Kondo , Hidemasa× Adachi, Motoyasu× Tsuda, Sakae× Shimizu, Rumi× Shibazaki, Chie× Adachi, Motoyasu |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Recently, polypentagonal water networks were observed in a protein capable of binding to ice crystals, or ice-binding protein (IBP). To examine such water networks and clarify their role in ice-binding, we determined X-ray crystal structures of a 65-residue defective isoform of an IBP (wild type, WT) and its 5 single mutants (A20L, A20G, A20T, A20V, and A20I). Polypentagonal water networks composed of approximately 50 semi-clathrate waters were solely observed on the strongest A20I mutant, which appeared to include a tetrahedral water cluster exhibiting perfect position match to the (10-10) 1st prism plane of a single ice crystal. Inclusion of another symmetrical water cluster in the polypentagonal network showed a perfect complementarity to the waters constructing the (20-21) pyramidal ice plane. The order of ice-binding strength was A20L < A20G < WT< A20T < A20V < A20I, where the top 3 mutants capable of binding to the 1st 12 prism and the pyramidal ice planes commonly contained a bifurcated γ-CH313 -group. These results suggest that a fine-tuning of the protein surface assisted by a side-chain group regulates the holding property of the polypentagonal water network, whose function is to freeze the host protein to specific ice planes. | |||||
| 書誌情報 |
Proceedings of the National Academy of Sciences of United States of America 巻 115, 号 21, p. 5456-5461, 発行日 2018-05 |
|||||
| 出版者 | ||||||
| 出版者 | National Academy of Sciences | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0027-8424 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1073/pnas.1800635115 | |||||
