{"created":"2023-05-15T14:38:02.868750+00:00","id":49084,"links":{},"metadata":{"_buckets":{"deposit":"a277dfeb-cc27-4a82-8e11-55f18cde80be"},"_deposit":{"created_by":1,"id":"49084","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"49084"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00049084","sets":["1"]},"author_link":["733972","733970","733973","733976","733971","733979","733974","733975","733969","733978","733980","733977"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-05","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"21","bibliographicPageEnd":"5461","bibliographicPageStart":"5456","bibliographicVolumeNumber":"115","bibliographic_titles":[{"bibliographic_title":"Proceedings of the National Academy of Sciences of United States of America "}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Recently, polypentagonal water networks were observed in a protein capable of binding to ice crystals, or ice-binding protein (IBP). To examine such water networks and clarify their role in ice-binding, we determined X-ray crystal structures of a 65-residue defective isoform of an IBP (wild type, WT) and its 5 single mutants (A20L, A20G, A20T, A20V, and A20I). Polypentagonal water networks composed of approximately 50 semi-clathrate waters were solely observed on the strongest A20I mutant, which appeared to include a tetrahedral water cluster exhibiting perfect position match to the (10-10) 1st prism plane of a single ice crystal. Inclusion of another symmetrical water cluster in the polypentagonal network showed a perfect complementarity to the waters constructing the (20-21) pyramidal ice plane. The order of ice-binding strength was A20L < A20G < WT< A20T < A20V < A20I, where the top 3 mutants capable of binding to the 1st 12 prism and the pyramidal ice planes commonly contained a bifurcated γ-CH313 -group. These results suggest that a fine-tuning of the protein surface assisted by a side-chain group regulates the holding property of the polypentagonal water network, whose function is to freeze the host protein to specific ice planes.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"National Academy of Sciences"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1073/pnas.1800635115","subitem_relation_type_select":"DOI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0027-8424","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Mahatabuddin , Sheikh"}],"nameIdentifiers":[{"nameIdentifier":"733969","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fukami , Daichi"}],"nameIdentifiers":[{"nameIdentifier":"733970","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Arai, Tatsuya"}],"nameIdentifiers":[{"nameIdentifier":"733971","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nishimiya, Yoshiyuki"}],"nameIdentifiers":[{"nameIdentifier":"733972","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shimizu, Rumi"}],"nameIdentifiers":[{"nameIdentifier":"733973","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shibazaki , Chie"}],"nameIdentifiers":[{"nameIdentifier":"733974","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kondo , Hidemasa"}],"nameIdentifiers":[{"nameIdentifier":"733975","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Adachi, Motoyasu"}],"nameIdentifiers":[{"nameIdentifier":"733976","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tsuda, Sakae"}],"nameIdentifiers":[{"nameIdentifier":"733977","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shimizu, Rumi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"733978","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shibazaki, Chie","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"733979","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Adachi, Motoyasu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"733980","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Polypentagonal ice-like water networks emerge solely in an activity-improved variant of ice-binding protein","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Polypentagonal ice-like water networks emerge solely in an activity-improved variant of ice-binding protein"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-06-12"},"publish_date":"2018-06-12","publish_status":"0","recid":"49084","relation_version_is_last":true,"title":["Polypentagonal ice-like water networks emerge solely in an activity-improved variant of ice-binding protein"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T07:56:32.984131+00:00"}