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Forced lipophagy reveals that lipid droplets are required for early embryonic development in mouse
https://repo.qst.go.jp/records/49020
https://repo.qst.go.jp/records/4902036a1c397-1523-4383-a64f-c893a5f54797
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2018-05-16 | |||||
| タイトル | ||||||
| タイトル | Forced lipophagy reveals that lipid droplets are required for early embryonic development in mouse | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
辰巳, 嵩征
× 辰巳, 嵩征× Takayama, Kaori× Ishii, Shunsuke× Yamamoto, Atsushi× Hara, Taichi× Minami, Naojiro× Miyasaka, Naoyuki× Kubota, Toshiro× Matsuura, Akira× Itakura, Eisuke× 塚本, 智史× 辰巳 嵩征× 塚本 智史 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Although autophagy is classically viewed as a non-selective degradation system, recent studies have revealed that various forms of selective autophagy also play crucial physiological roles. However, the induction of selective autophagy is not well understood. In this study, we established a forced selective autophagy system using a fusion of an autophagy adaptor and a substrate-binding protein. In both mammalian cells and fertilized mouse embryos, efficient forced lipophagy was induced by expression of a fusion of p62 (Sqstm1) and a lipid droplet (LD)-binding domain. In mouse embryos, induction of forced lipophagy caused a reduction in LD size and number, and decreased the triglyceride level throughout embryonic development, resulting in developmental retardation. Furthermore, lipophagy-induced embryos could eliminate excess LDs and were tolerant of lipotoxicity. Thus, by inducing forced lipophagy, expression of the p62 fusion protein generated LD-depleted cells, revealing an unexpected role of LD during preimplantation development. | |||||
| 書誌情報 |
Development 巻 145, 号 4, 発行日 2018-03 |
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| 出版者 | ||||||
| 出版者 | The Company of Biologists | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1242/dev.161893 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://dev.biologists.org/content/145/4/dev161893 | |||||
| 関連名称 | http://dev.biologists.org/content/145/4/dev161893 | |||||
