{"created":"2023-05-15T14:38:00.050485+00:00","id":49020,"links":{},"metadata":{"_buckets":{"deposit":"4bc34e9a-7144-4599-98c3-5d4de2578345"},"_deposit":{"created_by":1,"id":"49020","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"49020"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00049020","sets":["1"]},"author_link":["494371","494363","494368","494365","494370","494361","494372","494362","494366","494367","494369","494373","494364"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicVolumeNumber":"145","bibliographic_titles":[{"bibliographic_title":"Development"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Although autophagy is classically viewed as a non-selective degradation system, recent studies have revealed that various forms of selective autophagy also play crucial physiological roles. However, the induction of selective autophagy is not well understood. In this study, we established a forced selective autophagy system using a fusion of an autophagy adaptor and a substrate-binding protein. In both mammalian cells and fertilized mouse embryos, efficient forced lipophagy was induced by expression of a fusion of p62 (Sqstm1) and a lipid droplet (LD)-binding domain. In mouse embryos, induction of forced lipophagy caused a reduction in LD size and number, and decreased the triglyceride level throughout embryonic development, resulting in developmental retardation. Furthermore, lipophagy-induced embryos could eliminate excess LDs and were tolerant of lipotoxicity. Thus, by inducing forced lipophagy, expression of the p62 fusion protein generated LD-depleted cells, revealing an unexpected role of LD during preimplantation development.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"The Company of Biologists"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1242/dev.161893","subitem_relation_type_select":"DOI"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://dev.biologists.org/content/145/4/dev161893"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dev.biologists.org/content/145/4/dev161893","subitem_relation_type_select":"URI"}}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"辰巳, 嵩征"}],"nameIdentifiers":[{"nameIdentifier":"494361","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takayama, Kaori"}],"nameIdentifiers":[{"nameIdentifier":"494362","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ishii, Shunsuke"}],"nameIdentifiers":[{"nameIdentifier":"494363","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamamoto, Atsushi"}],"nameIdentifiers":[{"nameIdentifier":"494364","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hara, Taichi"}],"nameIdentifiers":[{"nameIdentifier":"494365","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Minami, Naojiro"}],"nameIdentifiers":[{"nameIdentifier":"494366","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Miyasaka, Naoyuki"}],"nameIdentifiers":[{"nameIdentifier":"494367","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kubota, Toshiro"}],"nameIdentifiers":[{"nameIdentifier":"494368","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Matsuura, Akira"}],"nameIdentifiers":[{"nameIdentifier":"494369","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Itakura, Eisuke"}],"nameIdentifiers":[{"nameIdentifier":"494370","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"塚本, 智史"}],"nameIdentifiers":[{"nameIdentifier":"494371","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"辰巳 嵩征","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"494372","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"塚本 智史","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"494373","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Forced lipophagy reveals that lipid droplets are required for early embryonic development in mouse","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Forced lipophagy reveals that lipid droplets are required for early embryonic development in mouse"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-05-16"},"publish_date":"2018-05-16","publish_status":"0","recid":"49020","relation_version_is_last":true,"title":["Forced lipophagy reveals that lipid droplets are required for early embryonic development in mouse"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:21:50.655420+00:00"}