WEKO3
アイテム
New nethod for estimating clustering of DNA lesions induced by physical/chemical mutagens using fluorescence anisotropy
https://repo.qst.go.jp/records/48497
https://repo.qst.go.jp/records/4849746a4933c-872d-4d2c-a322-e735f8c7c0c3
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2018-02-14 | |||||
| タイトル | ||||||
| タイトル | New nethod for estimating clustering of DNA lesions induced by physical/chemical mutagens using fluorescence anisotropy | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
赤松, 憲
× 赤松, 憲× 鹿園, 直哉× 齊藤, 毅× 赤松 憲× 鹿園 直哉 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We have developed a new method for estimating the localization of DNA damage such as apurinic/apyrimidinic sites (APs) on DNA using fluorescence anisotropy. This method is aimed at characterizing clustered DNA damage produced by DNA-damaging agents such as ionizing radiation and genotoxic chemicals. A fluorescent probe with an aminooxy group (AlexaFluor488) was used to label APs. We prepared a pUC19 plasmid with APs by heating under acidic conditions as a model for damaged DNA, and subsequently labeled the APs. We found that the observed fluorescence anisotropy (robs) decreases as averaged AP density (AP: number of APs per base pair) increases due to homo-FRET, and that the APs were randomly distributed. We applied this method to three DNA-damaging agents, 60Co -rays, methyl methanesulfonate (MMS), and neocarzinostatin (NCS). We found that robs–AP relationships differed significantly between MMS and NCS. At low AP density (AP < 0.001), the APs induced by MMS seemed to not be closely distributed, whereas those induced by NCS were remarkably clustered. In contrast, the AP clustering induced by 60Co -rays was similar to, but potentially more likely to occur than, random distribution. This simple method can be used to estimate mutagenicity of ionizing radiation and genotoxic chemicals. | |||||
| 書誌情報 |
Analytical Biochemistry 巻 536, p. 78-89, 発行日 2017-09 |
|||||
| 出版者 | ||||||
| 出版者 | Elsevier | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1016/j.ab.2017.08.007 | |||||
