{"created":"2023-05-15T14:37:35.816115+00:00","id":48497,"links":{},"metadata":{"_buckets":{"deposit":"df654ce2-fb94-4b03-82a0-3cfa6ef75e4c"},"_deposit":{"created_by":1,"id":"48497","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"48497"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00048497","sets":["1"]},"author_link":["487562","487563","487564","487565","487561"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-09","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"89","bibliographicPageStart":"78","bibliographicVolumeNumber":"536","bibliographic_titles":[{"bibliographic_title":"Analytical Biochemistry"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We have developed a new method for estimating the localization of DNA damage such as apurinic/apyrimidinic sites (APs) on DNA using fluorescence anisotropy. This method is aimed at characterizing clustered DNA damage produced by DNA-damaging agents such as ionizing radiation and genotoxic chemicals. A fluorescent probe with an aminooxy group (AlexaFluor488) was used to label APs. We prepared a pUC19 plasmid with APs by heating under acidic conditions as a model for damaged DNA, and subsequently labeled the APs. We found that the observed fluorescence anisotropy (robs) decreases as averaged AP density (AP: number of APs per base pair) increases due to homo-FRET, and that the APs were randomly distributed. We applied this method to three DNA-damaging agents, 60Co -rays, methyl methanesulfonate (MMS), and neocarzinostatin (NCS). We found that robs–AP relationships differed significantly between MMS and NCS. At low AP density (AP < 0.001), the APs induced by MMS seemed to not be closely distributed, whereas those induced by NCS were remarkably clustered. In contrast, the AP clustering induced by 60Co -rays was similar to, but potentially more likely to occur than, random distribution. This simple method can be used to estimate mutagenicity of ionizing radiation and genotoxic chemicals.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.ab.2017.08.007","subitem_relation_type_select":"DOI"}}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"赤松, 憲"}],"nameIdentifiers":[{"nameIdentifier":"487561","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鹿園, 直哉"}],"nameIdentifiers":[{"nameIdentifier":"487562","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"齊藤, 毅"}],"nameIdentifiers":[{"nameIdentifier":"487563","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"赤松 憲","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"487564","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鹿園 直哉","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"487565","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"New nethod for estimating clustering of DNA lesions induced by physical/chemical mutagens using fluorescence anisotropy","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"New nethod for estimating clustering of DNA lesions induced by physical/chemical mutagens using fluorescence anisotropy"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-02-14"},"publish_date":"2018-02-14","publish_status":"0","recid":"48497","relation_version_is_last":true,"title":["New nethod for estimating clustering of DNA lesions induced by physical/chemical mutagens using fluorescence anisotropy"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:27:25.054902+00:00"}