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Effects of carbon ion irradiation and X-ray irradiation on the ubiquitylated protein accumulation.
https://repo.qst.go.jp/records/48165
https://repo.qst.go.jp/records/48165162dae1f-b287-4b7d-be66-1c60b27b1f14
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2017-07-24 | |||||
| タイトル | ||||||
| タイトル | Effects of carbon ion irradiation and X-ray irradiation on the ubiquitylated protein accumulation. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Isozaki, Tetsuro
× Isozaki, Tetsuro× Fujita, Mayumi× Yamada, Shigeru× Imadome, Kaori× Shoji, Yoshimi× Yasuda, Takeshi× Nakayama, Fumiaki× Imai, Takashi× Matsubara, Hisahiro× 磯崎 哲朗× 藤田 真由美× 山田 滋× 今留 香織× 荘司 好美× 安田 武嗣× 中山 文明× 今井 高志× 松原 久裕 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | C-ion radiotherapy is associated with improved local control and survival in several types of tumors. Although C-ion irradiation is widely reported to effectively induce DNA damage in tumor cells, the effects of irradiation on proteins, such as protein stability or degradation in response to radiation stress, remain unknown. We aimed to compare the effects of C-ion and X-ray irradiation focusing on the cellular accumulation of ubiquitylated proteins. Cells from two human colorectal cancer cell lines, SW620 and SW480, were subjected to C-ion or X-ray irradiation and determination of ubiquitylated protein levels. High levels of ubiquitylated protein accumulation were observed in the C-ion-irradiated SW620 with a peak at 3 Gy; the accumulation was significantly lower in the X-ray-irradiated SW620 at all doses. Enhanced levels of ubiquitylated proteins were also detected in C-ion or X-ray-irradiated SW480, however, those levels were significantly lower than the peak detected in the C-ion-irradiated SW620. The levels of irradiation-induced ubiquitylated proteins decreased in a time-dependent manner, suggesting that the proteins were eliminated after irradiation. The treatment of C-ion-irradiated SW620 with a proteasome inhibitor (epoxomicin) enhanced the cell killing activity. The accumulated ubiquitylated proteins were co-localized with γ-H2AX, and with TP53BP1, in C-ion-irradiated SW620, indicating C-ion-induced ubiquitylated proteins may have some functions in the DNA repair system. Overall, we showed C-ion irradiation strongly induces the accumulation of ubiquitylated proteins in SW620. These characteristics may play a role in improving the therapeutic ratio of C-ion beams; blocking the clearance of ubiquitylated proteins may enhance sensitivity to C-ion radiation. | |||||
| 書誌情報 |
International journal of oncology 巻 49, 号 1, p. 144-152, 発行日 2016-07 |
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| 出版者 | ||||||
| 出版者 | D.A. Spandidos | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1019-6439 | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 27175736 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.3892/ijo.2016.3504 | |||||
